The anal pore route is efficient to infect Amblyomma spp. ticks with Rickettsia rickettsii and allows the assessment of the role played by infection control targets.

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  • Additional Information
    • Source:
      Publisher: Frontiers Media SA Country of Publication: Switzerland NLM ID: 101585359 Publication Model: eCollection Cited Medium: Internet ISSN: 2235-2988 (Electronic) Linking ISSN: 22352988 NLM ISO Abbreviation: Front Cell Infect Microbiol Subsets: MEDLINE
    • Publication Information:
      Original Publication: Lausanne : Frontiers Media SA
    • Subject Terms:
      Ticks*/microbiology ; Rocky Mountain Spotted Fever*/microbiology ; Ixodidae*/microbiology ; Rickettsia*; Animals ; Rabbits ; Rickettsia rickettsii ; Amblyomma ; Brazil/epidemiology
    • Abstract:
      Adult Amblyomma sculptum and Amblyomma aureolatum ticks are partially refractory to Rickettsia rickettsii when fed on infected hosts, hindering the functional characterization of potentially protective targets in the bacterial acquisition. In the current study, we used the anal pore route to infect adult A. sculptum and A. aureolatum ticks with R. rickettsii and to assess the effects of the knockdown of microplusin in infection control. The anal pore route was efficient to infect both species, resulting in a prevalence of around 100% of infected ticks. Higher loads of R. rickettsii were detected in microplusin-silenced A. aureolatum in relation to the control, as previously obtained when microplusin-silenced ticks were fed on R. rickettsii -infected rabbits. This is the first report showing R . rickettsii infection through the anal pore in Amblyomma ticks, highlighting this route as a powerful tool to assess the role played by additional targets in the control of pathogens.
      Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
      (Copyright © 2023 Nassar, Pavanelo, Labruna, Daffre, Esteves and Fogaça.)
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    • Contributed Indexing:
      Keywords: RNA interference; anal pore; immune factors; microplusin; rickettsiae; tick
    • Publication Date:
      Date Created: 20231030 Date Completed: 20231031 Latest Revision: 20231031
    • Publication Date:
      20231215
    • Accession Number:
      PMC10602902
    • Accession Number:
      10.3389/fcimb.2023.1260390
    • Accession Number:
      37900319