Bacteria associated with ovine gut parasites Trichuris ovis and Haemonchus contortus .

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  • Additional Information
    • Source:
      Publisher: London School Of Hygiene And Tropical Medicine Country of Publication: England NLM ID: 2985115R Publication Model: Electronic Cited Medium: Internet ISSN: 1475-2697 (Electronic) Linking ISSN: 0022149X NLM ISO Abbreviation: J Helminthol Subsets: MEDLINE
    • Publication Information:
      Publication: London : London School Of Hygiene And Tropical Medicine
      Original Publication: London [etc.]
    • Subject Terms:
      Haemonchus*/genetics ; Parasites*/genetics ; Nematoda*/genetics ; Sheep Diseases*/parasitology ; Haemonchiasis*/parasitology; Animals ; Sheep ; Trichuris ; RNA, Ribosomal, 16S/genetics ; Bacteria/genetics
    • Abstract:
      An associated microbiome of any host helps it in different metabolic processes ranging from the decomposition of food to the maturation of gametes. Organisms with a parasitic mode of life, though present at nutritious sites inside their host, maintain their own microbiome. Nevertheless, the comprehensive characterization and functionality of microbiome in parasitic organisms remain understudied. We selected two nematode parasites of Kashmir Merino sheep viz; Haemonchus contortus and Trichuris ovis based on their higher prevalence, difference in mode of nutrition, habitation site and effect on host. The objective of the study was to explore the bacteria associated with these parasitic nematodes of sheep. We adopted a 16S rRNA metagenomic sequencing approach to estimate and compare the bacterial communities present in these two nematode species. Nematode parasites from Kashmir Merino sheep were identified morphologically and confirmed with DNA characterization. H. contortus was dominated by phylum Proteobacteria (57%), Firmicutes (25%), Bacteroidota (15%) and Actinobacteriota (3%). Conversely, T. ovis showed Proteobacteria (78%) followed by Firmicutes (8%), Bacteroidota (8%), Actinobacteriota (1%), Fusobacteriota (1%) and other phyla (4%). This study provides a comprehensive account of the microbiome composition of H. contortus and T. ovis, both of which are highly prevalent among Kashmir Merino sheep. Additionally, T. ovis exhibited a greater bacterial diversity compared to H. contortus. Notably, these nematodes were found to harbor certain pathogenic bacteria. This study can further be carried forward in gaining insights into the complex relationship between the microbiota of a parasite and its pathogenicity, reproductive potential and host microbiome modification.
    • Contributed Indexing:
      Keywords: 16S rRNA metagenomics; Haemonchus contortus; Kashmir Merino sheep; Microbiome; Trichuris ovis; bacteria
    • Accession Number:
      0 (RNA, Ribosomal, 16S)
    • Publication Date:
      Date Created: 20231017 Date Completed: 20231023 Latest Revision: 20231129
    • Publication Date:
      20231215
    • Accession Number:
      10.1017/S0022149X23000573
    • Accession Number:
      37846203