Microbial P450 repertoire (P450ome) and its application feasibility in pharmaceutical industry, chemical industry, and environmental protection.

Publisher: Wiley Country of Publication: United States NLM ID: 7502021 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1097-0290 (Electronic) Linking ISSN: 00063592 NLM ISO Abbreviation: Biotechnol Bioeng Subsets: MEDLINE

Publication: <2005->: Hoboken, NJ : Wiley
Original Publication: New York, Wiley.

Protein Engineering* ; Chemical Industry*; Humans ; Conservation of Natural Resources ; Feasibility Studies ; Cytochrome P-450 Enzyme System/genetics ; Cytochrome P-450 Enzyme System/chemistry ; Drug Industry

Cytochrome P450s (CYPs) are heme-thiolated enzymes that catalyze the oxidation of C-H bonds in a regio- and stereo-selective manner. CYPs are widely present in the biological world. With the completion of more biological genome sequencing, the number and types of P450 enzymes have increased rapidly. P450 in microorganisms is easy to clone and express, rich in catalytic types, and strong in substrate adaptability, which has good application potential. Although the number of P450 enzymes found in microorganisms is huge, the function of most of the microorganism P450s has not been studied, and it contains a large number of excellent biocatalysts to be developed. This review is based on the P450 groups in microorganisms. First, it reviews the distribution of P450 groups in different microbial species, and then studies the application of microbial P450 enzymes in the pharmaceutical industry, chemical industry and environmental pollutant treatment in recent years. And focused on the application fields of P450 enzymes of different families to guide the selection of suitable P450s from the huge P450 library. In view of the current shortcomings of microbial P450 in the application process, the final solution is the most likely to assist the application of P450 enzymes in large-scale, that is, whole cell transformation combined with engineering, fusion P450 combined with immobilization technology.
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S&T Program of Hebei; B2021208018 Natural Science Foundation of Hebei Province

Keywords: P450ome; biocatalysis; cytochrome P450; environmental protection; pharmaceutics

9035-51-2 (Cytochrome P-450 Enzyme System)

Date Created: 20230928 Date Completed: 20231219 Latest Revision: 20240105

20240106

10.1002/bit.28565

37767638