Multiplexed discrimination of SARS-CoV-2 variants via plasmonic-enhanced fluorescence in a portable and automated device.

Publisher: Springer Nature Country of Publication: England NLM ID: 101696896 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2157-846X (Electronic) Linking ISSN: 2157846X NLM ISO Abbreviation: Nat Biomed Eng Subsets: MEDLINE

Publication: London : Springer Nature
Original Publication: [London] : Macmillan Publishers Limited, [2016]-

COVID-19*/diagnosis ; SARS-CoV-2*/genetics; Humans ; Biological Assay ; RNA ; Fluorescence

Portable assays for the rapid identification of lineages of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) are needed to aid large-scale efforts in monitoring the evolution of the virus. Here we report a multiplexed assay in a microarray format for the detection, via isothermal amplification and plasmonic-gold-enhanced near-infrared fluorescence, of variants of SARS-CoV-2. The assay, which has single-nucleotide specificity for variant discrimination, single-RNA-copy sensitivity and does not require RNA extraction, discriminated 12 lineages of SARS-CoV-2 (in three mutational hotspots of the Spike protein) and detected the virus in nasopharyngeal swabs from 1,034 individuals at 98.8% sensitivity and 100% specificity, with 97.6% concordance with genome sequencing in variant discrimination. We also report a compact, portable and fully automated device integrating the entire swab-to-result workflow and amenable to the point-of-care detection of SARS-CoV-2 variants. Portable, rapid, accurate and multiplexed assays for the detection of SARS-CoV-2 variants and lineages may facilitate variant-surveillance efforts.
(© 2023. The Author(s), under exclusive licence to Springer Nature Limited.)

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22274069 National Natural Science Foundation of China (National Science Foundation of China); JCYJ20180504165657443 Shenzhen Science and Technology Innovation Commission; JCYJ20210324104007020 Shenzhen Science and Technology Innovation Commission

63231-63-0 (RNA)

SARS-CoV-2 variants

Date Created: 20230922 Date Completed: 20231220 Latest Revision: 20231220

20231220

10.1038/s41551-023-01092-4

37735541