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Multiplexed discrimination of SARS-CoV-2 variants via plasmonic-enhanced fluorescence in a portable and automated device.
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- Author(s): Liu Y;Liu Y; Yang Y; Yang Y; Wang G; Wang G; Wang D; Wang D; Shao PL; Shao PL; Tang J; Tang J; He T; He T; Zheng J; Zheng J; Hu R; Hu R; Liu Y; Liu Y; Xu Z; Xu Z; Niu D; Niu D; Lv J; Lv J; Yang J; Yang J; Xiao H; Xiao H; Wu S; Wu S; He S; He S; Tang Z; Tang Z; Liu Y; Liu Y; Tang M; Tang M; Jiang X; Jiang X; Yuan J; Yuan J; Dai H; Dai H; Zhang B; Zhang B
- Source:
Nature biomedical engineering [Nat Biomed Eng] 2023 Dec; Vol. 7 (12), pp. 1636-1648. Date of Electronic Publication: 2023 Sep 21.- Publication Type:
Journal Article- Language:
English - Source:
- Additional Information
- Source: Publisher: Springer Nature Country of Publication: England NLM ID: 101696896 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2157-846X (Electronic) Linking ISSN: 2157846X NLM ISO Abbreviation: Nat Biomed Eng Subsets: MEDLINE
- Publication Information: Publication: London : Springer Nature
Original Publication: [London] : Macmillan Publishers Limited, [2016]- - Subject Terms:
- Abstract: Portable assays for the rapid identification of lineages of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) are needed to aid large-scale efforts in monitoring the evolution of the virus. Here we report a multiplexed assay in a microarray format for the detection, via isothermal amplification and plasmonic-gold-enhanced near-infrared fluorescence, of variants of SARS-CoV-2. The assay, which has single-nucleotide specificity for variant discrimination, single-RNA-copy sensitivity and does not require RNA extraction, discriminated 12 lineages of SARS-CoV-2 (in three mutational hotspots of the Spike protein) and detected the virus in nasopharyngeal swabs from 1,034 individuals at 98.8% sensitivity and 100% specificity, with 97.6% concordance with genome sequencing in variant discrimination. We also report a compact, portable and fully automated device integrating the entire swab-to-result workflow and amenable to the point-of-care detection of SARS-CoV-2 variants. Portable, rapid, accurate and multiplexed assays for the detection of SARS-CoV-2 variants and lineages may facilitate variant-surveillance efforts.
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- Accession Number: 63231-63-0 (RNA)
- Subject Terms: SARS-CoV-2 variants
- Publication Date: Date Created: 20230922 Date Completed: 20231220 Latest Revision: 20231220
- Publication Date: 20231220
- Accession Number: 10.1038/s41551-023-01092-4
- Accession Number: 37735541
- Source:
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