Bacteria intrinsic to Medicago sativa (alfalfa) reduce Salmonella enterica growth in planta.

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  • Additional Information
    • Source:
      Publisher: Oxford University Press Country of Publication: England NLM ID: 9706280 Publication Model: Print Cited Medium: Internet ISSN: 1365-2672 (Electronic) Linking ISSN: 13645072 NLM ISO Abbreviation: J Appl Microbiol Subsets: MEDLINE
    • Publication Information:
      Publication: 2022- : Oxford : Oxford University Press
      Original Publication: Oxford : Published for the Society for Applied Bacteriology by Blackwell Science, c1997-
    • Subject Terms:
      Salmonella enterica*/genetics ; Bacillaceae* ; Foodborne Diseases*; Animals ; Medicago sativa ; Salmonella typhimurium ; Vegetables
    • Abstract:
      Aims: The purpose of this study was to determine whether plant-associated bacteria (PAB) can reduce Salmonella enterica colonization and infection of alfalfa sprouts to reduce the risk of foodborne illness.
      Methods: We isolated PAB from alfalfa seeds and sprouts. Monoclonal isolates of the bacteria were obtained and tested for their ability to inhibit Salmonella Typhimurium growth in alfalfa sprouts over 6 days. Genome sequencing and annotation were used to construct draft genomes of the bacteria isolated in this study using Illumina sequencing platform.
      Results: We observed that a cocktail of five PAB could reduce Salmonella growth in alfalfa sprouts from ∼108 to ∼105 CFU g-1, demonstrating a protective role. Genome sequencing revealed that these bacteria were members of the Pseudomonas, Pantoea, and Priestia genus, and did not possess genes that were pathogenic to plants or animals.
      Conclusions: This work demonstrates that PAB can be utilized to reduce pathogen levels in fresh produce, which may be synergistic with other technologies to improve the safety of sprouts and other fresh produce.
      (© The Author(s) 2023. Published by Oxford University Press on behalf of Applied Microbiology International.)
    • Grant Information:
      University of Minnesota
    • Contributed Indexing:
      Keywords: Pantoea; Priestia; Pseudomonas; Salmonella; Food safety; alfalfa; biocontrol
    • Publication Date:
      Date Created: 20230905 Date Completed: 20230918 Latest Revision: 20230918
    • Publication Date:
      20240829
    • Accession Number:
      10.1093/jambio/lxad204
    • Accession Number:
      37669894