Liquid Transmission Electron Microscopy for Probing Collagen Biomineralization.

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  • Additional Information
    • Source:
      Publisher: American Chemical Society Country of Publication: United States NLM ID: 101088070 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1530-6992 (Electronic) Linking ISSN: 15306984 NLM ISO Abbreviation: Nano Lett Subsets: MEDLINE
    • Publication Information:
      Original Publication: Washington, DC : American Chemical Society, c2001-
    • Subject Terms:
      Biomineralization* ; Collagen*/chemistry; Extracellular Matrix ; Microscopy, Electron, Transmission ; Minerals
    • Abstract:
      Collagen biomineralization is fundamental to hard tissue assembly. While studied extensively, collagen mineralization processes are not fully understood, with the majority of theories derived from electron microscopy (EM) under static, dehydrated, or frozen conditions, unlike the liquid phase environment where mineralization occurs. Herein, novel liquid transmission EM (TEM) strategies are presented, in which collagen mineralization was explored in liquid for the first time via TEM. Custom thin-film enclosures were employed to visualize the mineralization of reconstituted collagen fibrils in a calcium phosphate and polyaspartic acid solution to promote intrafibrillar mineralization. TEM highlighted that at early time points precursor mineral particles attached to collagen and progressed to crystalline mineral platelets aligned with fibrils at later time points. This aligns with observations from other techniques and validates the liquid TEM approach. This work provides a new liquid imaging approach for exploring collagen biomineralization, advancing toward understanding disease pathogenesis and remineralization strategies for hard tissues.
    • Grant Information:
      PJT 180575 Canada CIHR
    • Contributed Indexing:
      Keywords: calcium phosphate; collagen mineralization; hard tissues; liquid electron microscopy; polyaspartic acid
    • Accession Number:
      9007-34-5 (Collagen)
      0 (Minerals)
    • Publication Date:
      Date Created: 20230905 Date Completed: 20231109 Latest Revision: 20231117
    • Publication Date:
      20240829
    • Accession Number:
      10.1021/acs.nanolett.3c02344
    • Accession Number:
      37669509