The cryo-EM 3D image reconstruction of isolated Lethocerus indicus Z-discs.

Publisher: Springer Netherlands Country of Publication: Netherlands NLM ID: 8006298 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1573-2657 (Electronic) Linking ISSN: 01424319 NLM ISO Abbreviation: J Muscle Res Cell Motil Subsets: MEDLINE

Publication: Dordrecht : Springer Netherlands
Original Publication: [London] Chapman and Hall.

Sarcomeres*/metabolism ; Actins*/metabolism; Animals ; Actinin/metabolism ; Muscle Proteins/metabolism ; Cryoelectron Microscopy ; Muscle, Skeletal/metabolism ; Solvents/metabolism ; Image Processing, Computer-Assisted

The Z-disk of striated muscle defines the ends of the sarcomere, which repeats many times within the muscle fiber. Here we report application of cryoelectron tomography and subtomogram averaging to Z-disks isolated from the flight muscles of the large waterbug Lethocerus indicus. We use high salt solutions to remove the myosin containing filaments and use gelsolin to remove the actin filaments of the A- and I-bands leaving only the thin filaments within the Z-disk which were then frozen for cryoelectron microscopy. The Lethocerus Z-disk structure is similar in many ways to the previously studied Z-disk of the honeybee Apis mellifera. At the corners of the unit cell are positioned trimers of paired antiparallel F-actins defining a large solvent channel, whereas at the trigonal positions are positioned F-actin trimers converging slowly towards their (+) ends defining a small solvent channel through the Z-disk. These near parallel F-actins terminate at different Z-heights within the Z-disk. The two types of solvent channel in Lethocerus are similar in size compared to those of Apis which are very different in size. Two types of α-actinin crosslinks were observed between oppositely oriented actin filaments. In one of these, the α-actinin long axis is almost parallel to the F-actins it crosslinks. In the other, the α-actinins are at a small but distinctive angle with respect to the crosslinked actin filaments. The utility of isolated Z-disks for structure determination is discussed.
(© 2023. The Author(s), under exclusive licence to Springer Nature Switzerland AG.)

J Muscle Res Cell Motil. 2017 Apr;38(2):241-250. (PMID: 28733815)
J Muscle Res Cell Motil. 1987 Dec;8(6):473-503. (PMID: 3443682)
Cell. 2014 Dec 4;159(6):1447-60. (PMID: 25433700)
Prog Biophys Mol Biol. 1982;39(3):183-231. (PMID: 6289376)
J Struct Biol. 2015 Nov;192(2):270-8. (PMID: 26455557)
Biophys J. 1998 Mar;74(3):1439-51. (PMID: 9512040)
Cell. 2021 Apr 15;184(8):2135-2150.e13. (PMID: 33765442)
J Biol Chem. 1988 Jun 5;263(16):7800-5. (PMID: 3286641)
Biophys J. 1971 Feb;11(2):204-10. (PMID: 5542613)
J Mol Biol. 1968 Jan 28;31(2):155-76. (PMID: 5635532)
J Mol Biol. 1974 Aug 25;87(4):671-83. (PMID: 4214935)
Proc Natl Acad Sci U S A. 2006 Mar 21;103(12):4451-6. (PMID: 16537423)
J Cell Biol. 1968 May;37(2):445-61. (PMID: 5656401)
J Cell Biol. 1978 Jun;77(3):837-52. (PMID: 28332)
Proc Natl Acad Sci U S A. 2019 Jul 30;116(31):15534-15539. (PMID: 31320587)
Elife. 2015 May 29;4:e06980. (PMID: 26023829)
EMBO J. 1993 Jul;12(7):2863-71. (PMID: 8335002)
J Struct Biol. 2000 Feb;129(1):1-16. (PMID: 10675292)
J Struct Biol. 2009 Apr;166(1):95-102. (PMID: 19263523)
Nat Commun. 2014 Sep 04;5:4808. (PMID: 25185801)
J Mol Biol. 1999 Jan 29;285(4):1549-62. (PMID: 9917396)
J Mol Biol. 1963 Sep;7:281-308. (PMID: 14064165)
J Struct Biol. 2005 Apr;150(1):109-21. (PMID: 15797735)
J Mol Biol. 2002 Sep 27;322(4):731-9. (PMID: 12270710)
J Mol Biol. 2012 Dec 14;424(5):248-56. (PMID: 23041423)
J Mol Biol. 1973 Sep 15;79(2):329-37. (PMID: 4760133)
J Mol Biol. 1985 Aug 20;184(4):621-43. (PMID: 4046026)
Int J Mol Sci. 2019 Apr 05;20(7):. (PMID: 30959804)
J Mol Biol. 2004 Apr 16;338(1):115-25. (PMID: 15050827)
J Microsc. 1982 Aug;127(Pt 2):127-38. (PMID: 7120365)
J Mol Biol. 2007 Apr 20;368(1):92-104. (PMID: 17331538)
J Cell Biol. 1987 Jul;105(1):29-39. (PMID: 3611188)
J Cell Sci. 1994 May;107 ( Pt 5):1115-29. (PMID: 7929622)
J Cell Biol. 1989 May;108(5):1775-82. (PMID: 2715177)
J Struct Biol. 2009 Dec;168(3):378-87. (PMID: 19732834)
Adv Exp Med Biol. 2000;481:207-18; discussion 219-20. (PMID: 10987074)
J Cell Biol. 1981 Aug;90(2):459-66. (PMID: 6793598)
Elife. 2023 Jan 16;12:. (PMID: 36645127)
J Muscle Res Cell Motil. 1992 Apr;13(2):183-9. (PMID: 1597512)
J Muscle Res Cell Motil. 2005;26(6-8):479-85. (PMID: 16450058)
J Cell Biol. 2001 Sep 3;154(5):1045-57. (PMID: 11535621)
PLoS One. 2010 Sep 09;5(9):. (PMID: 20844746)
J Cell Biol. 1990 Dec;111(6 Pt 2):2961-78. (PMID: 2269662)
Methods. 2016 Feb 15;95:78-85. (PMID: 26638773)
J Mol Med (Berl). 2006 Jun;84(6):446-68. (PMID: 16416311)
J Biol Chem. 2011 Mar 25;286(12):9897-904. (PMID: 21257757)
Ultramicroscopy. 2005 Aug;104(1):8-29. (PMID: 15935913)
Arch Biochem Biophys. 2015 Sep 1;581:78-85. (PMID: 25921875)
J Mol Biol. 1967 Jul 28;27(2):385-9. (PMID: 6048987)
Ultramicroscopy. 2006 Feb;106(3):240-54. (PMID: 16137829)
J Muscle Res Cell Motil. 1983 Feb;4(1):25-53. (PMID: 6841591)
J Mol Biol. 2006 Sep 1;361(5):823-38. (PMID: 16887144)
Curr Protoc Protein Sci. 2011 Aug;Chapter 17:Unit17.13. (PMID: 21842467)
J Cell Biol. 1989 May;108(5):1761-74. (PMID: 2715176)

S10 RR025080 United States RR NCRR NIH HHS; S10 OD018142 United States OD NIH HHS; R01 GM30598 United States GM NIGMS NIH HHS; R35 GM139616 United States GM NIGMS NIH HHS; S10 OD018142 United States CD ODCDC CDC HHS; R01 GM030598 United States GM NIGMS NIH HHS; R01 GM30598 United States GM NIGMS NIH HHS; S10 RR025080 United States RR NCRR NIH HHS; S10 OD018142 United States CD ODCDC CDC HHS

Keywords: Electron tomography; F-actin; Kettin; Projectin; α-Actinin

0 (Actins)
11003-00-2 (Actinin)
0 (Muscle Proteins)
0 (Solvents)

Date Created: 20230903 Date Completed: 20231124 Latest Revision: 20241202

20241204

PMC10843718

10.1007/s10974-023-09657-1

37661214