Mechanism and antibacterial synergies of poly(Dabco-BBAC) nanoparticles against multi-drug resistant Pseudomonas aeruginosa isolates from human burns.

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  • Additional Information
    • Source:
      Publisher: Elsevier Country of Publication: United States NLM ID: 1303703 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1090-2120 (Electronic) Linking ISSN: 00452068 NLM ISO Abbreviation: Bioorg Chem Subsets: MEDLINE
    • Publication Information:
      Publication: Amsterdam : Elsevier
      Original Publication: New York, London, Academic Press.
    • Subject Terms:
      Burns* ; COVID-19* ; Nanoparticles* ; Cystamine*/analogs & derivatives ; Cystamine*/pharmacology; Humans ; Anti-Bacterial Agents/pharmacology ; Gentamicins/pharmacology ; Microbial Sensitivity Tests ; Pseudomonas aeruginosa
    • Abstract:
      Multi-drug resistant bacteria are a major problem in the treatment of infectious diseases, such as pneumonia, meningitis, or even coronavirus disease 2019 (COVID-19). Cationic nanopolymers are a new type of antimicrobial agent with high efficiency. We synthesized and characterized cationic polymer based on 1,4-diazabicyclo [2.2.2] octane (DABCO) and Bis (bromoacetyl)cystamine (BBAC), named poly (DABCO-BBAC) nanoparticles(NPs), and produced 150 nm diameter NPs. The antibacterial activity of poly (DABCO-BBAC) against eight multi drug resistant (MDR) Pseudomonas aeruginosa isolates from human burns, its possible synergistic effect with gentamicin, and the mechanism of action were examined. Poly(DABCO-BBAC) could effectively inhibit and kill bacterial strains at a very low concentration calculated by minimum inhibitory concentration (MIC) assay. Nevertheless, its synergism index with gentamicin showed an indifferent effect. Moreover, transmission electron microscopy and lipid peroxidation assays showed that poly (DABCO-BBAC) distorted and damaged the bacterial cell wall. These results suggest that the poly (DABCO-BBAC) could be an effective antibacterial agent for MDR clinical pathogens.
      Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
      (Copyright © 2023. Published by Elsevier Inc.)
    • Grant Information:
      R01 AI050875 United States AI NIAID NIH HHS; R21 AI121700 United States AI NIAID NIH HHS
    • Contributed Indexing:
      Keywords: Antibacterial mechanism; Antibiotic synergism; Cationic nanopolymer; Multi-drug resistance, Poly(DABCO_BBAC) nanoparticles; Nanomedicine; Pseudomonas aeruginosa
    • Accession Number:
      0 (Anti-Bacterial Agents)
      0 (Gentamicins)
      X8M57R0JS5 (triethylenediamine)
      0 (bis(bromoacetyl)cystamine)
      R110LV8L02 (Cystamine)
    • Publication Date:
      Date Created: 20230811 Date Completed: 20230918 Latest Revision: 20240806
    • Publication Date:
      20240807
    • Accession Number:
      10.1016/j.bioorg.2023.106718
    • Accession Number:
      37566942