Effects of low-protein diet and feed restriction on mRNA expression of cationic amino acid transporters in porcine skeletal muscles.

Publisher: Wiley Country of Publication: Australia NLM ID: 100956805 Publication Model: Print Cited Medium: Internet ISSN: 1740-0929 (Electronic) Linking ISSN: 13443941 NLM ISO Abbreviation: Anim Sci J Subsets: MEDLINE

Publication: Richmond, Vic. : Wiley
Original Publication: Tokyo, Japan : Japanese Society of Zootechnical Science [1999-

Amino Acid Transport Systems, Basic*/genetics ; Amino Acid Transport Systems, Basic*/metabolism ; Lysine*/metabolism; Swine/genetics ; Animals ; Diet, Protein-Restricted/veterinary ; Diet/veterinary ; Arginine/metabolism ; Arginine/pharmacology ; Muscle, Skeletal/metabolism ; RNA, Messenger/genetics ; RNA, Messenger/metabolism ; Animal Feed/analysis

We investigated the effects of a low-protein diet and feed restriction on the mRNA expression of cationic amino acid transporters (CATs) in the longissimus dorsi (LD), rhomboideus (RH), and biceps femoris (BF) muscles of pigs. Eighteen piglets were divided into three groups: a control (CP21%), low-protein diet (LP, CP16%), and feed-restricted diet (FR, CP21%, 76% feed intake of control pigs) groups. The expression levels of CAT-1 in the LD and BF muscles of LP pigs were higher than that of control pigs, whereas that of FR pigs showed no difference. The CAT-2A expression levels in the RH muscle of FR pigs were higher than that of control pigs. The free lysine concentrations in all muscles of LP and FR pigs were lower than that of control pigs. To examine the factors that affect CATs mRNA expression, we evaluated the effects of lysine, arginine, insulin-like growth factor-I, and dexamethasone on the expression of CATs in C2C12 myotubes. CAT-1 expression levels increased in lysine and/or arginine deprivation. We show that CAT-1 and CAT-2A expression levels in skeletal muscles differ in response to dietary treatments and CAT-1 expression in skeletal muscles appears to increase in response to low free lysine concentrations.
(© 2023 Japanese Society of Animal Science.)

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17K15362 Ministry of Education, Science, and Culture, Japan; 26850170 Ministry of Education, Science, and Culture, Japan

Keywords: cationic amino acid transporter; feed restriction; low-protein; pig; skeletal muscle

0 (Amino Acid Transport Systems, Basic)
K3Z4F929H6 (Lysine)
94ZLA3W45F (Arginine)
0 (RNA, Messenger)

Date Created: 20230808 Date Completed: 20231102 Latest Revision: 20231102

20240829

10.1111/asj.13861

37551564