Impact of prepubertal bovine ovarian tissue pre-freeze holding duration on follicle quality.

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  • Additional Information
    • Source:
      Publisher: Elsevier Urban & Partner Country of Publication: Poland NLM ID: 101160559 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2300-732X (Electronic) Linking ISSN: 1642431X NLM ISO Abbreviation: Reprod Biol Subsets: MEDLINE
    • Publication Information:
      Publication: <2013- > : Olsztyn, Poland : Elsevier Urban & Partner
      Original Publication: Olsztyn, Poland : Polish Society for Biology of Reproduction, c2001-
    • Subject Terms:
      Ovarian Follicle* ; Fertility Preservation*/methods; Female ; Animals ; Cattle ; Humans ; Infant ; Freezing ; Ovary/pathology ; Cryopreservation/veterinary ; Cryopreservation/methods
    • Abstract:
      Ovarian tissue cryopreservation prior to gonadotoxic treatment is the only recommended option for fertility preservation in prepubertal girls. Due to the technical complexity of this technique, limited number of centres across the world are equipped to offer the facility. Hence, the retrieved ovarian tissue needs to be maintained at hypothermic temperature (4 °C) for long time during shipment. The time taken between tissue retrieval and cryopreservation could influence the functionality of cells during fertility restoration. This study explored the tissue integrity and follicle quality of ovarian cortical slices subjected to pre-freeze holding for various time durations in vitro. Prepubertal bovine ovarian tissue from < 12 months old animals were handled at hypothermic holding (4 °C) for 0, 24, 48 and 72 h. The tissues were assessed for follicle viability through confocal analysis of live-dead labelled samples, and follicle quality and tissue integrity through histology. Results have shown that follicle viability, and overall follicle quality were not significantly affected at the end of 72 h hypothermic holding. Though, the observation reassures extended hypothermic holding prior to freezing, findings need to be validated in human tissue prior to use in clinical fertility preservation programs.
      Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
      (Copyright © 2023 Society for Biology of Reproduction & the Institute of Animal Reproduction and Food Research of Polish Academy of Sciences in Olsztyn. Published by Elsevier B.V. All rights reserved.)
    • Contributed Indexing:
      Keywords: fertility preservation; follicle quality; hypothermic holding; ovarian tissue cryopreservation
    • Publication Date:
      Date Created: 20230802 Date Completed: 20230828 Latest Revision: 20230828
    • Publication Date:
      20230828
    • Accession Number:
      10.1016/j.repbio.2023.100794
    • Accession Number:
      37531931