Leishmania donovani persistence and circulation causing cutaneous leishmaniasis in unusual-foci of Nepal.

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  • Additional Information
    • Source:
      Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN: 2045-2322 (Electronic) Linking ISSN: 20452322 NLM ISO Abbreviation: Sci Rep Subsets: MEDLINE
    • Publication Information:
      Original Publication: London : Nature Publishing Group, copyright 2011-
    • Subject Terms:
      Leishmania donovani*/genetics ; Leishmaniasis, Cutaneous*/epidemiology ; Dermatitis*; Humans ; Nepal/epidemiology ; DNA, Kinetoplast/genetics
    • Abstract:
      Cutaneous leishmaniasis cases have increased dramatically in recent years in Nepal. The study offers molecular identification of the Leishmania species using 40 patient's aspiration biopsy samples, targeting markers kinetoplast minicircle DNA (kDNA) and internal transcribed spacer-1 (ITS1). Among molecularly diagnosed 22 cutaneous leishmaniasis cases, L. donovani complex was identified in 13 instances and L. major in 9 cases. The ITS1 PCR was positive in 12 of the positive nested- kDNA PCR cases (12/22), confirming L. donovani complex in seven of the cases and L. major in five of the cases. In addition, the study conclude that concurrent occurrence of atypical cutaneous infections caused by L. donovani parasite in 59.1% of cases and typical cutaneous infections caused by L. major parasite in 40.9% of cases. A Phylogentic analaysis showed that the detected L. donovani species present null genetic distances from seven references of L. donovani, but slight differences between ITS1 sequences and not grouped into a significant monophyletic cluster.
      (© 2023. The Author(s).)
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    • Accession Number:
      0 (DNA, Kinetoplast)
    • Publication Date:
      Date Created: 20230729 Date Completed: 20230731 Latest Revision: 20230802
    • Publication Date:
      20231215
    • Accession Number:
      PMC10387047
    • Accession Number:
      10.1038/s41598-023-37458-6
    • Accession Number:
      37516780