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The comparison of gut gene expression and bacterial community in Diaphorina citri (Hemiptera: Liviidae) adults fed on Murraya exotica and 'Shatangju' mandarin (Citrus reticulate cv. Shatangju).
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- Additional Information
- Source:
Publisher: BioMed Central Country of Publication: England NLM ID: 100965258 Publication Model: Electronic Cited Medium: Internet ISSN: 1471-2164 (Electronic) Linking ISSN: 14712164 NLM ISO Abbreviation: BMC Genomics Subsets: MEDLINE
- Publication Information:
Original Publication: London : BioMed Central, [2000-
- Subject Terms:
- Abstract:
Background: Diaphorina citri Kuwayama is an important citrus pest. It serves as the vector for the transmission of Candidatus Liberibacter asiaticus (CLas), which induced a destructive disease, Huanglongbing, and caused huge economic losses. During the interaction between insects and plants, insects have evolved a series of mechanisms to adapt to various host plants. Murraya exotica and 'Shatangju' mandarin (Citrus reticulate cv. Shatangju) are the Rutaceae species from different genera that have been discovered as suitable hosts for D. citri adults. While the adaptation mechanism of this pest to these two host plants is unclear.
Results: In this study, RNA-seq and 16 S rDNA amplification sequencing were performed on the gut of D. citri adults reared on M. exotica and 'Shatangju' mandarin. RNA-seq results showed that a total of 964 differentially expressed genes were found in different gut groups with two host plant treatments. The impacted genes include those that encode ribosomal proteins, cathepsins, and mitochondrial respiratory chain complexes. According to 16 S rDNA sequencing, the compositions of the gut bacterial communities were altered by different treatments. The α and β diversity analyses confirmed that the host plant changes influenced the gut microbial diversity. The functional classification analysis by Tax4Fun revealed that 27 KEGG pathways, mostly those related to metabolism, including those for nucleotide metabolism, energy metabolism, metabolism of cofactors and vitamins, amino acid metabolism, carbohydrate metabolism, xenbiotics biodegradation and metabolism, lipid metabolism, and biosynthesis of other secondary metabolites, were significantly altered.
Conclusion: Our preliminary findings shed light on the connection between D. citri and host plants by showing that host plants alter the gene expression profiles and bacterial community composition of D. citri adults.
(© 2023. The Author(s).)
- References:
Insect Sci. 2022 Nov 8;:. (PMID: 36346663)
Front Microbiol. 2021 Jun 02;12:670383. (PMID: 34149656)
BMC Genomics. 2020 Aug 6;21(1):546. (PMID: 32762647)
Insect Sci. 2019 Aug;26(4):671-682. (PMID: 29286204)
Insect Biochem Mol Biol. 2014 Jul;50:58-67. (PMID: 24727019)
Annu Rev Entomol. 2015 Jan 7;60:17-34. (PMID: 25341109)
Int J Mol Sci. 2021 Jun 25;22(13):. (PMID: 34202141)
Insects. 2022 Aug 02;13(8):. (PMID: 36005319)
Res Microbiol. 2002 Oct;153(8):503-9. (PMID: 12437211)
Pest Manag Sci. 2018 Dec;74(12):2738-2746. (PMID: 29726075)
Mol Ecol. 2021 Jan;30(2):499-516. (PMID: 33219534)
Environ Microbiol. 2013 Jul;15(7):1956-68. (PMID: 23078522)
Nat Commun. 2015 Jul 14;6:7618. (PMID: 26173063)
J Exp Biol. 2021 Jan 28;224(Pt 2):. (PMID: 33509844)
Environ Int. 2020 Oct;143:105886. (PMID: 32623217)
Gene. 2020 Feb 15;727:144246. (PMID: 31759988)
Curr Opin Insect Sci. 2020 Oct;41:79-85. (PMID: 32823202)
Mol Ecol. 2012 Dec;21(24):6134-51. (PMID: 23017151)
Int J Mol Sci. 2020 Aug 30;21(17):. (PMID: 32872681)
Microb Ecol. 2020 Aug;80(2):410-422. (PMID: 32052099)
J Econ Entomol. 2021 Apr 13;114(2):597-610. (PMID: 33547790)
Bioinformatics. 2018 Sep 1;34(17):i884-i890. (PMID: 30423086)
Trends Ecol Evol. 2020 Mar;35(3):259-277. (PMID: 31791830)
Front Microbiol. 2019 Aug 23;10:1948. (PMID: 31507561)
Insects. 2021 Nov 25;12(12):. (PMID: 34940142)
Front Microbiol. 2020 Jun 30;11:1366. (PMID: 32714300)
Plant Signal Behav. 2016 Nov;11(11):e1249080. (PMID: 27763819)
J Appl Microbiol. 2007 Dec;103(6):2664-75. (PMID: 17973916)
Annu Rev Entomol. 2015 Jan 7;60:233-52. (PMID: 25341101)
Mol Ecol. 2022 Aug;31(15):3999-4016. (PMID: 35665559)
Mol Ecol. 2022 Feb;31(3):723-735. (PMID: 34837439)
Microbiol Resour Announc. 2020 Jun 18;9(25):. (PMID: 32554788)
Curr Opin Insect Sci. 2015 Jun;9:62-68. (PMID: 32846710)
ISME J. 2020 Mar;14(3):847-856. (PMID: 31896788)
Pest Manag Sci. 2021 Jul;77(7):3216-3223. (PMID: 33751790)
Cold Spring Harb Perspect Biol. 2022 Feb 1;14(2):. (PMID: 34312250)
Mol Ecol. 2014 Mar;23(6):1433-1444. (PMID: 24350573)
Insect Sci. 2020 Oct;27(5):895-907. (PMID: 30924288)
FEMS Microbiol Rev. 2013 Sep;37(5):699-735. (PMID: 23692388)
mSystems. 2020 Mar 17;5(2):. (PMID: 32184361)
Comput Struct Biotechnol J. 2020 Nov 11;18:3395-3401. (PMID: 33294135)
Sci Rep. 2018 Jun 25;8(1):9620. (PMID: 29942001)
Trends Microbiol. 2009 Dec;17(12):529-35. (PMID: 19853457)
BMC Genomics. 2021 Mar 16;22(1):189. (PMID: 33726671)
J Insect Physiol. 2014 Dec;71:21-9. (PMID: 25257538)
- Grant Information:
2022SDZG06 the open competition program of top ten critical priorities of Agricultural Science and Technology Innovation for the 14th Five-Year Plan of Guangdong Province; 2021ZDJS001 Guangdong Province key construction discipline Scientific Research Capacity Improvement Program; 2019A1515110878 Basic and Applied Basic Research Foundation of Guangdong Province
- Contributed Indexing:
Keywords: 16S rDNA; Diaphorina citri; Differentially expressed genes; Gut bacterial community; Host plants; RNA-seq
- Accession Number:
0 (DNA, Ribosomal)
- Subject Terms:
Diaphorina citri
- Publication Date:
Date Created: 20230724 Date Completed: 20230726 Latest Revision: 20230727
- Publication Date:
20230727
- Accession Number:
PMC10364414
- Accession Number:
10.1186/s12864-023-09308-2
- Accession Number:
37488494
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