Compositional variations in metal nanoparticle components of welding fumes impact lung epithelial cell toxicity.

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  • Additional Information
    • Source:
      Publisher: Taylor & Francis Country of Publication: England NLM ID: 100960995 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1528-7394 (Print) Linking ISSN: 00984108 NLM ISO Abbreviation: J Toxicol Environ Health A Subsets: MEDLINE
    • Publication Information:
      Original Publication: Washington, D.C. ; London : Taylor & Francis, c1998-
    • Subject Terms:
      Welding* ; Metal Nanoparticles*/toxicity ; Iron*/toxicity ; Manganese*/toxicity ; Occupational Exposure* ; Lung*/pathology; Humans ; A549 Cells ; Electrodes ; Reactive Oxygen Species/analysis ; Cation Transport Proteins/genetics ; Inflammation/chemically induced ; Cytokines/analysis ; Chemokines/analysis ; Transferrin/analysis
    • Abstract:
      Welding fumes contain harmful metals and gas by-products associated with development of lung dysfunction, asthma, bronchitis, and lung cancer. Two prominent welding fume particulate metal components are nanosized iron (Fe) and manganese (Mn) which might induce oxidative stress and inflammation resulting in pulmonary injury. Welding fume toxicity may be dependent upon metal nanoparticle (NP) components. To examine toxicity of welding fume NP components, a system was constructed for controlled and continuous NP generation from commercial welding and customized electrodes with varying proportions of Fe and Mn. Aerosols generated consisted of nanosized particles and were compositionally consistent with each electrode. Human alveolar lung A459 epithelial cells were exposed to freshly generated metal NP mixtures at a target concentration of 100 µg/m 3 for 6 hr and then harvested for assessment of cytotoxicity, generation of reactive oxygen species (ROS), and alterations in the expression of genes and proteins involved in metal regulation, inflammatory responses, and oxidative stress. Aerosol exposures decreased cell viability and induced increased ROS production. Assessment of gene expression demonstrated variable up-regulation in cellular mechanisms related to metal transport and storage, inflammation, and oxidative stress based upon aerosol composition. Specifically, interleukin-8 (IL-8) demonstrated the most robust changes in both transcriptional and protein levels after exposure. Interleukin-8 has been determined to serve as a primary cytokine mediating inflammatory responses induced by welding fume exposures in alveolar epithelial cells. Overall, this study demonstrated variations in cellular responses to metal NP mixtures suggesting compositional variations in NP content within welding fumes may influence inhalation toxicity.
    • Grant Information:
      T42 OH008455 United States OH NIOSH CDC HHS
    • Contributed Indexing:
      Keywords: Welding fumes; air-liquid interface exposure; composition; inflammation; metal oxide; metal storage; metal transport; nanoparticles mixtures; oxidative stress
    • Accession Number:
      E1UOL152H7 (Iron)
      42Z2K6ZL8P (Manganese)
      0 (Reactive Oxygen Species)
      0 (Cation Transport Proteins)
      0 (Cytokines)
      0 (Chemokines)
      0 (Transferrin)
    • Publication Date:
      Date Created: 20230724 Date Completed: 20230906 Latest Revision: 20230920
    • Publication Date:
      20231215
    • Accession Number:
      10.1080/15287394.2023.2238209
    • Accession Number:
      37485994