Cell-loaded genipin cross-linked collagen/gelatin skin substitute adorned with zinc-doped bioactive glass-ceramic for cutaneous wound regeneration.

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  • Additional Information
    • Source:
      Publisher: Elsevier Country of Publication: Netherlands NLM ID: 7909578 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-0003 (Electronic) Linking ISSN: 01418130 NLM ISO Abbreviation: Int J Biol Macromol Subsets: MEDLINE
    • Publication Information:
      Publication: Amsterdam : Elsevier
      Original Publication: Guildford, Eng., IPC Science and Technology Press.
    • Subject Terms:
      Gelatin*/chemistry ; Ceramics*/chemistry ; Ceramics*/pharmacology ; Wound Healing*/drug effects ; Zinc*/chemistry ; Zinc*/pharmacology ; Collagen*/chemistry ; Tissue Scaffolds*/chemistry ; Fibroblasts*/drug effects ; Iridoids*/chemistry ; Iridoids*/pharmacology; Animals ; Mice ; Skin, Artificial ; Skin/drug effects ; Skin/injuries ; Mice, Inbred BALB C ; Biocompatible Materials/chemistry ; Biocompatible Materials/pharmacology ; Cell Proliferation/drug effects ; Nanocomposites/chemistry ; Tissue Engineering/methods
    • Abstract:
      An optimal tissue-engineered dermal substitute should possess biocompatibility and cell adhesion conduction to facilitate fibroblast and keratinocyte infiltration and proliferation, as well as angiogenesis potential to escalate wound healing. Zinc was doped to bioactive glass-ceramic (Zn-BGC) to promote biocompatibility and angiogenesis properties. Zn-BGC was then incorporated into a collagen (Col) and gelatin (Gel) porous scaffold. The bioactive porous bionanocomposite exhibited biocompatibility along with improved cell attachment and proliferation. Scaffolds including Col-Gel/Zn-BGC with or without mouse embryonic fibroblasts were applied on full-thickness skin wounds on the BALB/c mice to assess their wound healing potential in vivo. The results indicated that the biodegradation rate of the Col-Gel/Zn-BGC nanocomposites was comparable to the rate of skin tissue regeneration in vivo. Macroscopic wound healing results showed that Col-Gel/Zn-BGC loaded with mouse embryonic fibroblast possesses the smallest wound size, indicating the fastest healing process. Histopathological evaluations displayed that the optimal wound regeneration was observed in Col-Gel/Zn-BGC nanocomposites loaded with mouse embryonic fibroblasts indicated by epithelialization and angiogenesis; besides the number of fibroblasts and hair follicles was increased. The bioactive nanocomposite scaffold of Col-Gel containing Zn-BGC nanoparticles loaded with mouse embryonic fibroblasts can be employed as a desirable skin substitute to ameliorate cutaneous wound regeneration.
      Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
      (Copyright © 2023. Published by Elsevier B.V.)
    • Contributed Indexing:
      Keywords: Glass-ceramic nanoparticles; Skin substitute; Wound healing; Zinc
    • Accession Number:
      9000-70-8 (Gelatin)
      J41CSQ7QDS (Zinc)
      9007-34-5 (Collagen)
      A3V2NE52YG (genipin)
      0 (Iridoids)
      85422-94-2 (Glass ceramics)
      0 (Biocompatible Materials)
    • Publication Date:
      Date Created: 20230721 Date Completed: 20240525 Latest Revision: 20241105
    • Publication Date:
      20241105
    • Accession Number:
      10.1016/j.ijbiomac.2023.125898
    • Accession Number:
      37479201