Parental Folate Deficiency Inhibits Proliferation and Increases Apoptosis of Neural Stem Cells in Rat Offspring: Aggravating Telomere Attrition as a Potential Mechanism.

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  • Additional Information
    • Source:
      Publisher: MDPI Publishing Country of Publication: Switzerland NLM ID: 101521595 Publication Model: Electronic Cited Medium: Internet ISSN: 2072-6643 (Electronic) Linking ISSN: 20726643 NLM ISO Abbreviation: Nutrients Subsets: MEDLINE
    • Publication Information:
      Original Publication: Basel, Switzerland : MDPI Publishing
    • Subject Terms:
      Telomerase* ; Folic Acid Deficiency* ; Neural Stem Cells*; Animals ; Rats ; Telomere Shortening ; Telomere ; Folic Acid/pharmacology ; Apoptosis ; Cell Proliferation
    • Abstract:
      The effect of maternal folate status on the fetal central nervous system (CNS) is well recognized, while evidence is emerging that such an association also exists between fathers and offspring. The biological functions of telomeres and telomerase are also related to neural cell proliferation and apoptosis. The study aimed to investigate the effect of parental folate deficiency on the proliferation and apoptosis of neural stem cells (NSCs) in neonatal offspring and the role of telomeres in this effect. In this study, rats were divided into four groups: maternal folate-deficient and paternal folate-deficient diet (D-D) group; maternal folate-deficient and paternal folate-normal diet (D-N) group; maternal folate-normal and paternal folate-deficient diet (N-D) group; and the maternal folate-normal and paternal folate-normal diet (N-N) group. The offspring were sacrificed at postnatal day 0 (PND0), and NSCs were cultured from the hippocampus and striatum tissues of offspring for future assay. The results revealed that parental folate deficiency decreased folate levels, increased homocysteine (Hcy) levels of the offspring's brain tissue, inhibited proliferation, increased apoptosis, shortened telomere length, and aggravated telomere attrition of offspring NSCs in vivo and in vitro. In vitro experiments further showed that offspring NSCs telomerase activity was inhibited due to parental folate deficiency. In conclusion, parental folate deficiency inhibited the proliferation and increased apoptosis of offspring NSCs, maternal folate deficiency had more adverse effects than paternal, and the mechanisms may involve the telomere attrition of NSCs.
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    • Grant Information:
      82003439 National Natural Science Foundation of China; 2019KJ166 Scientific Research Program of Tianjin Municipal Education Commission; 2022KJ203 Scientific Research Program of Tianjin Municipal Education Commission
    • Contributed Indexing:
      Keywords: apoptosis; folate; neural stem cells; parental; proliferation; telomere attrition
    • Accession Number:
      EC 2.7.7.49 (Telomerase)
      935E97BOY8 (Folic Acid)
    • Publication Date:
      Date Created: 20230714 Date Completed: 20230717 Latest Revision: 20230718
    • Publication Date:
      20240829
    • Accession Number:
      PMC10343379
    • Accession Number:
      10.3390/nu15132843
    • Accession Number:
      37447170