V3: an enigmatic isoform of the proteoglycan versican.

Publisher: American Physiological Society Country of Publication: United States NLM ID: 100901225 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1522-1563 (Electronic) Linking ISSN: 03636143 NLM ISO Abbreviation: Am J Physiol Cell Physiol Subsets: MEDLINE

Original Publication: Bethesda, Md. : American Physiological Society,

Alternative Splicing* ; Versicans*/genetics; Extracellular Matrix ; Protein Isoforms/genetics ; Humans

V3 is an isoform of the extracellular matrix (ECM) proteoglycan (PG) versican generated through alternative splicing of the versican gene such that the two major exons coding for sequences in the protein core that support chondroitin sulfate (CS) glycosaminoglycan (GAG) chain attachment are excluded. Thus, versican V3 isoform carries no GAGs. A survey of PubMed reveals only 50 publications specifically on V3 versican, so it is a very understudied member of the versican family, partly because to date there are no antibodies that can distinguish V3 from the CS-carrying isoforms of versican, that is, to facilitate functional and mechanistic studies. However, a number of in vitro and in vivo studies have identified the expression of the V3 transcript during different phases of development and in disease, and selective overexpression of V3 has shown dramatic phenotypic effects in "gain and loss of function" studies in experimental models. Thus, we thought it would be useful and instructive to discuss the discovery, characterization, and the putative biological importance of the enigmatic V3 isoform of versican.

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R01 EB012558 United States EB NIBIB NIH HHS; R01 AI130280 United States AI NIAID NIH HHS; 16539 United Kingdom VAC_ Versus Arthritis; U19 AI125378 United States AI NIAID NIH HHS; 14871 United Kingdom VAC_ Versus Arthritis; G0501816 United Kingdom MRC_ Medical Research Council; R01 DK096087 United States DK NIDDK NIH HHS; 76445 United Kingdom MRC_ Medical Research Council; R41 HL106967 United States HL NHLBI NIH HHS; 18472 United Kingdom VAC_ Versus Arthritis

Keywords: atherosclerosis; cell proliferation; hyaluronan; inflammation; versican

0 (Protein Isoforms)
126968-45-4 (Versicans)
0 (VCAN protein, human)

Date Created: 20230703 Date Completed: 20230809 Latest Revision: 20240802

20250114

PMC10511178

10.1152/ajpcell.00059.2023

37399500