[Differential Expression of a Foreign Gene in Arabidopsis Mitochondria In Organelle].

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  • Additional Information
    • Source:
      Publisher: Izdatelstvo Nauka Country of Publication: Russia (Federation) NLM ID: 0105454 Publication Model: Print Cited Medium: Print ISSN: 0026-8984 (Print) Linking ISSN: 00268984 NLM ISO Abbreviation: Mol Biol (Mosk) Subsets: MEDLINE
    • Publication Information:
      Original Publication: Moskva : Izdatelstvo Nauka
    • Subject Terms:
      Arabidopsis*/genetics ; Arabidopsis*/metabolism ; Arabidopsis Proteins*/genetics ; Arabidopsis Proteins*/metabolism; Mitochondria/genetics ; Mitochondria/metabolism ; Promoter Regions, Genetic ; DNA/metabolism ; Gene Expression Regulation, Plant
    • Abstract:
      Genetic transformation of higher eukaryote mitochondria in vivo is an unresolved and important problem. For efficient expression of foreign genetic material in mitochondria, it is necessary to select regulatory elements that provide a high level of transcription and transcript stability. This work is aimed at studying the effectiveness of regulatory elements of mitochondrial genes flanking exogenous DNA using the phenomenon of natural competence of plant mitochondria. For this purpose, genetic constructs carrying the GFP gene under the control of the promoter regions of the RRN26 or COX1 genes and one of the two 3'-untranslated regions (3'-UTR) of mitochondrial genes were imported into isolated Arabidopsis mitochondria, followed by transcription in organello. It was shown that the level of GFP expression under the control of promoters of the RRN26 or COX1 genes in organello correlates with the level of transcription of these genes observed in vivo. At the same time, the presence of the tRNA^(Trp) sequence in the 3'-UTR leads to a higher level of the GFP transcript than the presence in this region of the 3'-UTR of the NAD4 gene containing the binding site of the MTSF1 protein. The results we obtained open prospects for creating a system for efficient transformation of the mitochondrial genome.
    • Contributed Indexing:
      Keywords: Arabidopsis thaliana mitochondria; DNA import; GFP; RPOTmp; in organello; promoter region; regulation of gene expression; untranslated region
    • Accession Number:
      0 (Arabidopsis Proteins)
      9007-49-2 (DNA)
    • Publication Date:
      Date Created: 20230616 Date Completed: 20230619 Latest Revision: 20230619
    • Publication Date:
      20240829
    • Accession Number:
      10.31857/S002689842303014X, EDN: CHUECL
    • Accession Number:
      37326049