A Human Cell-based Assay to Assess the Induction of Vasculature Formation for Non-genotoxic Carcinogenicity Testing Purposes: A Pilot Study.

Item request has been placed! ×
Item request cannot be made. ×
loading   Processing Request
Read More Add to Saved list
  • Additional Information
    • Source:
      Publisher: SAGE Publications Country of Publication: England NLM ID: 8110074 Publication Model: Print-Electronic Cited Medium: Print ISSN: 0261-1929 (Print) Linking ISSN: 02611929 NLM ISO Abbreviation: Altern Lab Anim Subsets: MEDLINE
    • Publication Information:
      Publication: 2019- : [London] : SAGE Publications
      Original Publication: London : Fund for the Replacement of Animals in Medical Experiments, [c1981-
    • Subject Terms:
      Carcinogens*; Humans ; Pilot Projects ; Carcinogenicity Tests/methods
    • Abstract:
      The induction of vasculature formation is proposed to be a significant mechanism behind the non-genotoxic carcinogenicity of a chemical. The vasculature formation model used in this study is based on the coculture of human primary HUVECs and hASCs. This model was used to develop an assay to assess the induction of vasculature formation. Three assay protocols, based on different conditions, were developed and compared in order to identify the optimal conditions required. Some serum supplements and growth factors were observed to be essential for initiating vasculature formation. Of the studied putative positive reference chemicals, aspartame, sodium nitrite, bisphenol A and nicotine treatment led to a clear induction of vasculature formation, but arsenic and cadmium treatment only led to a slight increase. This human cell-based assay has the potential to be used as one test within a next generation testing battery, to assess the non-genotoxic carcinogenicity of a chemical through the mechanism of vasculature formation induction.
    • Contributed Indexing:
      Keywords: angiogenesis; in vitro model; non-genotoxic carcinogenicity; vascular model; vascular toxicity; vasculature formation; vasculogenesis
    • Accession Number:
      0 (Carcinogens)
    • Publication Date:
      Date Created: 20230501 Date Completed: 20230605 Latest Revision: 20230605
    • Publication Date:
      20231215
    • Accession Number:
      10.1177/02611929231171165
    • Accession Number:
      37125451