Development of a rapid detection method for enrofloxacin in food.

Item request has been placed! ×
Item request cannot be made. ×
loading   Processing Request
Read More Add to Saved list
  • Additional Information
    • Source:
      Publisher: Taylor & Francis Country of Publication: England NLM ID: 8510274 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2046-5556 (Electronic) Linking ISSN: 02648725 NLM ISO Abbreviation: Biotechnol Genet Eng Rev Subsets: MEDLINE
    • Publication Information:
      Publication: <2014- > : Abingdon : Taylor & Francis
      Original Publication: Newcastle upon Tyne : Intercept, 1984-
    • Subject Terms:
      Enrofloxacin*/analysis ; Enzyme-Linked Immunosorbent Assay*/methods; Animals ; Fluoroquinolones/analysis ; Food Analysis/methods ; Anti-Bacterial Agents/analysis ; Food Contamination/analysis ; Limit of Detection
    • Abstract:
      Develop the ic-ELISA rapid detection method of Enrofloxacin (ENR). Corresponding antibodies are obtained by animal immunity to identify their titer and specificity. The optimal coating time was obtained by indirect competition ELISA, and the antigen coating time, suitable coating concentration, primary antibody dilution factor, blocking solution blocking time, primary antibody reaction time and secondary antibody reaction time were optimized, and the specificity and accuracy of the method were evaluated. The ic-ELISA rapid detection method of ENR, IC 50 was 9.13 ng/mL, and the linear detection range (IC 20 -IC 80 ) was 4.16-20.03 ng/mL. The LOD limit is 2.11 ng/mL. The cross-reactivity rate of 9 fluoroquinolones was above 10%, and the average recovery rate was above 80%. The reason why the heterologous coating is more sensitive may be due to the fact that the piperazine group of ofloxacin is one less carbon atom than enrofloxacin, and ofloxacin is connected to the main ring by N and O hybridization, while enrofloxacin is connected to the main ring through a ternary ring, these two reasons may cause the charge density of extracyclic oxygen at the ofloxacin binding site to be higher than that of enrofloxacin, and the binding ability to antibodies is stronger. Therefore, when heterologous coating, the competitive inhibition rate against enrofloxacin is higher and the effect is better. The conclusion obtained through this experiment is that the detection method has strong broad spectrum and good sensitivity, and can quickly detect the total amount of enrofloxacin and its seven common fluoroquinolones in fish and eggs.
    • Contributed Indexing:
      Keywords: Enrofloxacin; heterologous coating; homologous coating; indirect competitive ELISA
    • Accession Number:
      3DX3XEK1BN (Enrofloxacin)
      0 (Fluoroquinolones)
      0 (Anti-Bacterial Agents)
    • Publication Date:
      Date Created: 20230421 Date Completed: 20241029 Latest Revision: 20241029
    • Publication Date:
      20241029
    • Accession Number:
      10.1080/02648725.2023.2204701
    • Accession Number:
      37083187