Microbial degradation of organochlorine pesticide: 2,4-Dichlorophenoxyacetic acid by axenic and mixed consortium.

Item request has been placed! ×
Item request cannot be made. ×
loading   Processing Request
Read More Add to Saved list
  • Additional Information
    • Source:
      Publisher: Elsevier Applied Science Country of Publication: England NLM ID: 9889523 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-2976 (Electronic) Linking ISSN: 09608524 NLM ISO Abbreviation: Bioresour Technol Subsets: MEDLINE
    • Publication Information:
      Original Publication: Barking, Essex, England : New York, N.Y. : Elsevier Applied Science ; Elsevier Science Pub. Co., 1991-
    • Subject Terms:
      Herbicides* ; Pesticides*/metabolism ; Soil Pollutants*/analysis ; Soil Pollutants*/metabolism ; Arthrobacter*/metabolism; Humans ; Ecosystem ; Biodegradation, Environmental ; Microbial Consortia ; 2,4-Dichlorophenoxyacetic Acid/metabolism ; Soil Microbiology
    • Abstract:
      The presence of 2,4-dichlorophenoxyacetic acid (2,4-D), an organochlorine herbicide, in the environment has raised public concern as it poses hazard to both humans and the ecosystem. Three potential strains having the capability to degrade 2,4-D were isolated from on site agricultural soil and identified as Arthrobacter sp. SVMIICT25, Sphingomonas sp. SVMIICT11 and Stenotrophomonas sp. SVMIICT13. Over 12 days of incubation, 81-90% of 100 mg/L of 2,4-D degradation was observed at 2% inoculum. A shorter lag phase with 80% of degradation efficiency was observed within 5 days when the inoculum size was increased to 10%. Six microbial consortia were prepared by combining the isolates along with in-house strains, Bacillus sp. and Pseudomonas sp. Consortia R3 (Arthrobacter sp. + Sphingomonas sp.), operated with 10% of inoculum, showed 85-90% degradation within 4 days and 98-100% in 9 days. Further, targeted exo-metabolite analysis confirmed the presence and catabolism of intermediate 2,4-dichlorophenol and 4-chlorophenol compounds.
      Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
      (Copyright © 2023 Elsevier Ltd. All rights reserved.)
    • Contributed Indexing:
      Keywords: 16S rRNA gene; Bioremediation; Co-metabolism; Dehalogenation; Ultra-performance liquid chromatography (UPLC)
    • Accession Number:
      0 (Herbicides)
      0 (Pesticides)
      0 (Soil Pollutants)
      2577AQ9262 (2,4-Dichlorophenoxyacetic Acid)
    • Publication Date:
      Date Created: 20230410 Date Completed: 20230605 Latest Revision: 20230605
    • Publication Date:
      20240829
    • Accession Number:
      10.1016/j.biortech.2023.129031
    • Accession Number:
      37037331