Ferrocene tagged primary antibody generates electrochemical signal: An electrochemical immunosensing platform for the monitoring of vitamin D deficiency in clinical samples.

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  • Additional Information
    • Source:
      Publisher: Elsevier Country of Publication: Netherlands NLM ID: 7909578 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-0003 (Electronic) Linking ISSN: 01418130 NLM ISO Abbreviation: Int J Biol Macromol Subsets: MEDLINE
    • Publication Information:
      Publication: Amsterdam : Elsevier
      Original Publication: Guildford, Eng., IPC Science and Technology Press.
    • Subject Terms:
      Vitamin D Deficiency* ; Biosensing Techniques*/methods ; Graphite*/chemistry ; Metal Nanoparticles*/chemistry; Humans ; Immunoassay/methods ; Metallocenes ; Reproducibility of Results ; Antibodies ; Electrochemical Techniques/methods ; Biomarkers ; Gold/chemistry ; Limit of Detection
    • Abstract:
      In this paper, a new kind of ultrasensitive and low-cost electrochemical immunosensing probe was designed to monitor vitamin D deficiency using 25(OH)D 3 as a clinical biomarker. Ferrocene carbaldehyde conjugated on Ab-25(OH)D 3 antibodies was used as an electrochemical probe for generating signals. The graphene nanoribbon-modified electrode (GNRs) was used to immobilize the (Ab-25(OH)D 3 -Fc) conjugate. The high electron transferability, greater surface area, and effective biocompatibility of GNRs enabled the capture of the greater number of primary antibodies (Ab-25(OH)D 3 ). The developed probe was structurally and morphologically characterized. The step-wise modification was investigated by electrochemical techniques. The direct electrochemistry of ferrocene enabled 25(OH)D 3 biomarker detection with excellent sensitivity. The reduction in peak current was proportional to the concentrations of 25(OH)D 3 in the range of 1-100 ng mL -1 with a 0.1 ng mL -1 limit of detection. The probe was tested in terms of reproducibility, repeatability, and stability. Finally, the developed immunosensing probe was applied in serum samples for 25(OH)D 3 quantification, and no significant difference was noticed in the assay results when compared with the standard chemiluminescent immunoassay (CLIA) method. The developed detection strategy has a wider scope for future potential clinical diagnostics applications.
      Competing Interests: Declaration of competing interest Authors declare no conflict of interest.
      (Copyright © 2023 Elsevier B.V. All rights reserved.)
    • Contributed Indexing:
      Keywords: 25-Hydroxy vitamin D(3); And clinical validation; Differential pulse voltammetry; Electrochemical immunosensor; Ferrocene-carbaldehyde; Graphene nanoribbons (GNRs)
    • Accession Number:
      U96PKG90JQ (ferrocene)
      0 (Metallocenes)
      0 (Antibodies)
      0 (Biomarkers)
      7440-57-5 (Gold)
      7782-42-5 (Graphite)
    • Publication Date:
      Date Created: 20230401 Date Completed: 20230519 Latest Revision: 20230519
    • Publication Date:
      20230519
    • Accession Number:
      10.1016/j.ijbiomac.2023.124269
    • Accession Number:
      37003374