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Viral metagenomics unveils MW (Malawi) polyomavirus infection in Brazilian pediatric patients with acute respiratory disease.
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- Author(s): da Silva AS;da Silva AS; de Campos GM; de Campos GM; Giovanetti M; Giovanetti M; Giovanetti M; Giovanetti M; Zucherato VS; Zucherato VS; Lima ARJ; Lima ARJ; Santos EV; Santos EV; Haddad R; Haddad R; Ciccozzi M; Ciccozzi M; Carlos Júnior Alcantara L; Carlos Júnior Alcantara L; Carlos Júnior Alcantara L; Elias MC; Elias MC; Sampaio SC; Sampaio SC; Covas DT; Covas DT; Covas DT; Kashima S; Kashima S; Slavov SN; Slavov SN; Slavov SN
- Source:
Journal of medical virology [J Med Virol] 2023 Apr; Vol. 95 (4), pp. e28688.- Publication Type:
Journal Article; Research Support, Non-U.S. Gov't- Language:
English - Source:
- Additional Information
- Source: Publisher: Wiley-Liss Country of Publication: United States NLM ID: 7705876 Publication Model: Print Cited Medium: Internet ISSN: 1096-9071 (Electronic) Linking ISSN: 01466615 NLM ISO Abbreviation: J Med Virol Subsets: MEDLINE
- Publication Information: Publication: New York Ny : Wiley-Liss
Original Publication: New York, Liss. - Subject Terms:
- Abstract: Viral metagenomics has been extensively applied for the identification of emerging or poorly characterized viruses. In this study, we applied metagenomics for the identification of viral infections among pediatric patients with acute respiratory disease, but who tested negative for SARS-CoV-2. Twelve pools composed of eight nasopharyngeal specimens were submitted to viral metagenomics. Surprisingly, in two of the pools, we identified reads belonging to the poorly characterized Malawi polyomavirus (MWPyV). Then, the samples composing the positive pools were individually tested using quantitative polymerase chain reaction for identification of the MWPyV index cases. MWPyV-positive samples were also submitted to respiratory virus panel testing due to the metagenomic identification of different clinically important viruses. Of note, MWPyV-positive samples tested also positive for respiratory syncytial virus types A and B. In this study, we retrieved two complete MWPyV genome sequences from the index samples that were submitted to phylogenetic inference to investigate their viral origin. Our study represents the first molecular and genomic characterization of MWPyV obtained from pediatric patients in South America. The detection of MWPyV in acutely infected infants suggests that this virus might participate (coparticipate) in cases of respiratory symptoms. Nevertheless, future studies based on testing of a larger number of clinical samples and MWPyV complete genomes appear to be necessary to elucidate if this emerging polyomavirus might be clinically important.
(© 2023 Wiley Periodicals LLC.) - References: Siebrasse EA, Reyes A, Lim ES, et al. Identification of MW polyomavirus, a novel polyomavirus in human stool. J Virol. 2012;86:10321-10326.
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Torres C, Barrios ME, Cammarata RV, et al. High diversity of human polyomaviruses in environmental and clinical samples in Argentina: detection of JC, BK, Merkel-cell, Malawi, and human 6 and 7 polyomaviruses. Sci Total Environ. 2016;542:192-202.
Rockett RJ, Bialasiewicz S, Mhango L, et al. Acquisition of human polyomaviruses in the first 18 months of life. Emerging Infect Dis. 2015;21:365-367.
Rockett RJ, Sloots TP, Bowes S, et al. Detection of novel polyomaviruses, TSPyV, HPyV6, HPyV7, HPyV9 and MWPyV in feces, urine, blood, respiratory swabs and cerebrospinal fluid. PLoS One. 2013;8:e62764.
Nicol JTJ, Leblond V, Arnold F, et al. Seroprevalence of human Malawi polyomavirus. J Clin Microbiol. 2014;52:321-323.
Kamminga S, Meijden E, Brouwer C, Feltkamp M, Zaaijer H. Prevalence of DNA of fourteen human polyomaviruses determined in blood donors. Transfusion. 2019;59:3689-3697. - Contributed Indexing: Keywords: MWPyV; Malawi polyomavirus; acute respiratory symptoms; pediatric patients; viral metagenomics
- Publication Date: Date Created: 20230322 Date Completed: 20230517 Latest Revision: 20230622
- Publication Date: 20231215
- Accession Number: 10.1002/jmv.28688
- Accession Number: 36946498
- Source:
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