Clearance of bacteria from lymph nodes in sheep immunized with Brucella suis S2 vaccine is associated with M1 macrophage activation.

Publisher: BioMed Central Country of Publication: England NLM ID: 9309551 Publication Model: Electronic Cited Medium: Internet ISSN: 1297-9716 (Electronic) Linking ISSN: 09284249 NLM ISO Abbreviation: Vet Res Subsets: MEDLINE

Publication: 2011- : London : BioMed Central
Original Publication: Paris : Editions Scientifiques Elsevier ; INRA, c1993-

Brucella melitensis* ; Brucella suis* ; Brucella Vaccine* ; Brucellosis*/prevention & control ; Brucellosis*/veterinary ; Sheep Diseases*/prevention & control; Animals ; Lymph Nodes ; Macrophage Activation ; Macrophages ; Sheep ; Vaccines, Attenuated

Ovine brucellosis is a global zoonotic disease of sheep caused by Brucella melitensis, which inflicts a significant burden on human and animal health. Brucella suis strain S2 (B. suis S2) is a smooth live attenuated vaccine for the prevention of ovine brucellosis in China. However, no previous studies have assessed the immunogenicity of B. suis S2 vaccine after oral immunization in sheep. Here, we attempted to evaluate the ovine immune response over the course of B. suis S2 immunization and to identify in vivo predictors for vaccine development. Body temperature, serum Brucella antibodies, serum cytokines (IL-12p70 and interferon [IFN]-γ), and bacterial load in the mandibular lymph nodes (LN), superficial cervical LN, superficial inguinal LN, and spleen were investigated to determine the safety and efficacy of the vaccine. The abnormal body temperature of sheep occurred within 8 days post-infection (dpi). Brucella suis S2 persisted for a short time (< 21 dpi) in the mandibular LN. The highest level of IL-12p70 was observed at 9 dpi, whereas serum IFN-γ levels peaked at 12 dpi. Transcriptome analysis and quantitative reverse transcription PCR were performed to determine gene expression profiles in the mandibular LN of sheep. Antigen processing and presentation pathway was the dominant pathway related to the dataset. Our studies suggest that the immune response in ovine LN resembled type 1 immunity with the secretion of IL-12p70 and IFN-γ after B.suis S2 immunization and the vaccine may eliminate Brucella via stimulation of M1 macrophages through the course of Th cells.
(© 2023. The Author(s).)

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No. YSPTZX202013 Academician Innovation Platform Project of Hainan Province; ZY2022HN09 Special Funding Projects for Local Science and Technology Development Guided by the Central Committee; 32160831 Natural Science Foundation of China

Keywords: Brucella; lymphatic nodes; macrophages; sheep; vaccine

0 (Brucella Vaccine)
0 (Vaccines, Attenuated)

Date Created: 20230315 Date Completed: 20230322 Latest Revision: 20230322

20240829

PMC10013293

10.1186/s13567-023-01147-z

36918910