In Vivo Tracking of Dendritic Cell Migration.

Publisher: Humana Press Country of Publication: United States NLM ID: 9214969 Publication Model: Print Cited Medium: Internet ISSN: 1940-6029 (Electronic) Linking ISSN: 10643745 NLM ISO Abbreviation: Methods Mol Biol Subsets: MEDLINE

Publication: Totowa, NJ : Humana Press
Original Publication: Clifton, N.J. : Humana Press,

Dendritic Cells* ; Proteins*; Animals ; Mice ; Cell Movement ; Antigens ; Adaptive Immunity ; Lymph Nodes ; Mice, Inbred C57BL

Dendritic cells (DCs) in peripheral tissue serve as a sentinel to invasion and maintain tolerance. They ingest and carry antigens to the draining lymph nodes and present antigens to antigen-specific T cells to initiate acquired immune responses. Thus, understanding DC migration from peripheral tissues and function is critical for understanding DCs' roles in immune homeostasis. Here, we introduced the KikGR in vivo photolabeling system, an ideal tool for monitoring precise cellular movements and related functions in vivo under physiological conditions and during various immune responses that occur in pathologic condition. Using a mouse line expressing photoconvertible fluorescent protein KikGR, we can label DCs in peripheral tissues by changing the color of KikGR from green to red after exposure to violet light and accurately track DC migration from each peripheral tissue to its respective draining lymph nodes.
(© 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.)

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Keywords: Dendritic cells; In vivo photo labeling; KikGR mouse; Migration; Photoconvertible protein; Tracking

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Date Created: 20230311 Date Completed: 20230314 Latest Revision: 20230424

20230424

10.1007/978-1-0716-2938-3_3

36905507