Draft genomes of Blastocystis subtypes from human samples of Colombia.

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  • Additional Information
    • Source:
      Publisher: BioMed Central Country of Publication: England NLM ID: 101462774 Publication Model: Electronic Cited Medium: Internet ISSN: 1756-3305 (Electronic) Linking ISSN: 17563305 NLM ISO Abbreviation: Parasit Vectors Subsets: MEDLINE
    • Publication Information:
      Original Publication: London : BioMed Central
    • Subject Terms:
      Blastocystis*/genetics ; Blastocystis Infections*; Animals ; Humans ; Colombia ; Genetic Variation ; Phylogeny ; DNA, Protozoan/genetics ; Feces
    • Abstract:
      Background: Blastocystis is one of the most common eukaryotic microorganisms colonizing the intestines of both humans and animals, but the conditions under which it may be a pathogen are unclear.
      Methods: To study the genomic characteristics of circulating subtypes (ST) in Colombia, we established nine xenic cultures from Blastocystis isolated from human fecal samples, we identified 10 different subtypes, since one sample had a mixed infection. Thus, the genomes of the subtypes ST1 (n = 3), ST2 (n = 1), ST3 (n = 2), ST6 (n = 1), ST7 (n = 1), and ST8 (n = 2) were sequenced using Illumina and Oxford Nanopore Technologies (ONT).
      Results: Analyses of these draft nuclear genomes indicated remarkable diversity in terms of genome size and guanine-cytosine (GC) content among the compared STs. Illumina sequencing-only draft genomes contained 824 to 2077 scaffolds, with total genome size ranging from 12 to 13.2 Mb and N 50 values ranging from 10,585 to 29,404 base pairs (bp). The genome of one ST1 isolate was sequenced using ONT. This assembly was more contiguous, with a size of 20 million base pairs (Mb) spread over 116 scaffolds, and an N 50 of 248,997 bp.
      Conclusion: This work represents one of the few large-scale comparative genomic analyses of Blastocystis isolates, providing an additional glimpse into its genomic diversity.
      (© 2023. The Author(s).)
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    • Grant Information:
      Internal funds Universidad del Rosario; Internal funds Universidad del Rosario
    • Contributed Indexing:
      Keywords: Blastocystis; Comparative genomics; Genomic diversity; Nanopore sequencing
    • Accession Number:
      0 (DNA, Protozoan)
    • Publication Date:
      Date Created: 20230203 Date Completed: 20230206 Latest Revision: 20230207
    • Publication Date:
      20230207
    • Accession Number:
      PMC9896827
    • Accession Number:
      10.1186/s13071-022-05619-7
    • Accession Number:
      36732768