Minimizing the Lag Phase of Cupriavidus necator Growth under Autotrophic, Heterotrophic, and Mixotrophic Conditions.

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  • Author(s): Amer A;Amer A;Amer A; Kim Y; Kim Y
  • Source:
    Applied and environmental microbiology [Appl Environ Microbiol] 2023 Feb 28; Vol. 89 (2), pp. e0200722. Date of Electronic Publication: 2023 Jan 31.
  • Publication Type:
    Journal Article; Research Support, Non-U.S. Gov't
  • Language:
    English
  • Additional Information
    • Source:
      Publisher: American Society for Microbiology Country of Publication: United States NLM ID: 7605801 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1098-5336 (Electronic) Linking ISSN: 00992240 NLM ISO Abbreviation: Appl Environ Microbiol Subsets: MEDLINE
    • Publication Information:
      Original Publication: Washington, American Society for Microbiology.
    • Subject Terms:
      Cupriavidus necator*/metabolism; Heterotrophic Processes ; Carbon Dioxide/metabolism ; Glycerol/metabolism ; Autotrophic Processes/physiology ; Acetates/metabolism
    • Abstract:
      Cupriavidus necator has the unique metabolic capability to grow under heterotrophic, autotrophic, and mixotrophic conditions. In the current work, we examined the effect of growth conditions on the metabolic responses of C. necator. In our lab-scale experiments, autotrophic growth was rapid, with a short lag phase as the exponential growth stage was initiated in 6 to 12 h. The lag phase extended significantly (>22 h) at elevated O 2 and CO 2 partial pressures, while the duration of the lag phase was independent of the H 2 or N 2 partial pressure. Under heterotrophic conditions with acetate as the organic substrate, the lag phase length was short (<12 h), but it increased with increasing acetate concentrations. When glucose and glycerol were provided as the organic substrate, the lag phase was consistently long (>12 h) regardless of the examined substrate concentrations (up to 10.0 g/L). In the transition experiments, C. necator cells showed rapid transitions from autotrophic to heterotrophic growth in less than 12 h and vice versa. Our experimental results indicate that C. necator can rapidly grow with both autotrophic and heterotrophic substrates, while the lag time substantially increases with nonacetate organic substrates (e.g., glucose or glycerol), high acetate concentrations, and high O 2 and CO 2 partial pressures. IMPORTANCE The current work investigated the inhibition of organic and gaseous substrates on the microbial adaption of Cupriavidus necator under several metabolic conditions commonly employed for commercial polyhydroxyalkanoate production. We also proposed a two-stage cultivation system to minimize the lag time required to change over between the heterotrophic, autotrophic, and mixotrophic pathways.
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    • Contributed Indexing:
      Keywords: PHA-accumulating bacteria; Ralstonia eutropha; inhibition by high substrate concentration; lag phase; mixotrophic growth
    • Accession Number:
      142M471B3J (Carbon Dioxide)
      PDC6A3C0OX (Glycerol)
      0 (Acetates)
    • Publication Date:
      Date Created: 20230131 Date Completed: 20230302 Latest Revision: 20230801
    • Publication Date:
      20240829
    • Accession Number:
      PMC9972949
    • Accession Number:
      10.1128/aem.02007-22
    • Accession Number:
      36719244