Protocol for the generation of human induced hepatic stem cells using Sendai virus vectors.

Item request has been placed! ×
Item request cannot be made. ×
loading   Processing Request
Read More Add to Saved list
  • Additional Information
    • Source:
      Publisher: Cell Press Country of Publication: United States NLM ID: 101769501 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2666-1667 (Electronic) Linking ISSN: 26661667 NLM ISO Abbreviation: STAR Protoc Subsets: MEDLINE
    • Publication Information:
      Original Publication: [Cambridge, MA] : Cell Press, [2020]-
    • Subject Terms:
      Cellular Reprogramming* ; Induced Pluripotent Stem Cells*; Humans ; Sendai virus/genetics ; Kruppel-Like Factor 4 ; Hepatocytes
    • Abstract:
      Our recent study demonstrated the generation of induced tissue-specific stem/progenitor (iTS/iTP) cells by the transient overexpression of reprogramming factors combined with tissue-specific selection. Here, we present a protocol to reprogram human hepatocytes to generate human induced tissue-specific liver stem (iTS-L) cells. Human hepatocytes are transfected with Sendai virus vectors (SeV) expressing OCT3/4, SOX2, KLF4, and c-MYC. iTS-L cells continuously express mRNA of hepatocyte-specific markers (HNF1β and HNF4α) and do not form teratomas. For complete details on the use and execution of this protocol, please refer to Nakashima et al. (2022). 1 .
      Competing Interests: Declaration of interests The authors declare no competing interests.
      (Copyright © 2022 The Author(s). Published by Elsevier Inc. All rights reserved.)
    • References:
      Stem Cell Reports. 2020 May 12;14(5):909-923. (PMID: 32302553)
      Brief Funct Genomics. 2013 May;12(3):164-73. (PMID: 23585580)
      iScience. 2022 Sep 05;25(10):105052. (PMID: 36147945)
      Eur Heart J. 2013 Sep;34(33):2618-29. (PMID: 22798560)
      Mol Ther Methods Clin Dev. 2019 Jan 29;13:243-252. (PMID: 30828587)
      Stem Cells Dev. 2018 Jul 1;27(13):871-887. (PMID: 29737235)
      Cell. 2007 Nov 30;131(5):861-72. (PMID: 18035408)
      Nature. 2010 Sep 16;467(7313):285-90. (PMID: 20644535)
      Science. 2014 Aug 15;345(6198):822-5. (PMID: 25035411)
    • Contributed Indexing:
      Keywords: Cell Differentiation; Cell culture; Health Sciences; Stem Cells
    • Accession Number:
      0 (Kruppel-Like Factor 4)
    • Publication Date:
      Date Created: 20230103 Date Completed: 20230105 Latest Revision: 20230125
    • Publication Date:
      20240829
    • Accession Number:
      PMC9700318
    • Accession Number:
      10.1016/j.xpro.2022.101884
    • Accession Number:
      36595898