Detection of unusual Cryptosporidium parvum subtype in patients with gastrointestinal cancer in Egypt.

Publisher: Springer International Country of Publication: Germany NLM ID: 8703571 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1432-1955 (Electronic) Linking ISSN: 09320113 NLM ISO Abbreviation: Parasitol Res Subsets: MEDLINE

Original Publication: Berlin : Springer International, c1987-

Cryptosporidium parvum*/genetics ; Cryptosporidiosis*/epidemiology ; Cryptosporidium*/genetics ; Scrapie* ; Gastrointestinal Neoplasms*/epidemiology; Animals ; Sheep/genetics ; Humans ; Male ; Egypt/epidemiology ; Feces ; Polymerase Chain Reaction ; Glycoproteins/genetics ; Genotype

While the importance of cryptosporidiosis in immunocompromised persons is well known, the prevalence of Cryptosporidium spp. in cancer patients is not clear. The current study was designed to assess the occurrence and genetic characteristics of Cryptosporidium spp. in patients with gastrointestinal (GI) cancer in Egypt. Stool samples were collected from 100 patients with GI malignancies and 20 healthy individuals without any GI manifestations (control group). They were screened by microscopy and the immunochromatographic RIDA®QUICK Cryptosporidium kit. Subtyping of Cryptosporidium spp. was conducted by sequence analysis of the glycoprotein 60 (gp60) locus. Sociodemographic, environmental data and information on GI symptoms, cancer types, and clinical treatment were obtained via a questionnaire. By microscopy and RIDA®QUICK, only 7% (7/100) of GI cancer patients were positive for Cryptosporidium, compared with 40% (40/100) by gp60 nPCR. No positives were obtained from the control group. Male sex (P = 0.02) and younger age (P = 0.004) were major Cryptosporidium risk factors for infection. The occurrence of Cryptosporidium was also significantly more frequent (P = 0.003) in watery stool samples. Sequence analysis of the gp60 amplicons (~ 400 bp) identified a novel C. parvum subtype with nine TCA repeats and eleven ACATCA repeats. A formal subtype designation could not be made due to the short sequence length. More studies should be conducted to verify the common occurrence of this unusual C. parvum subtype and establish its genetic identity.
(© 2022. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)

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111 Project (D20008) Ministry of Education of China

Keywords: Cryptosporidiosis; GI cancer patients; Genotyping; Microscopy; RIDA®QUICK; gp60

0 (Glycoproteins)

Date Created: 20221220 Date Completed: 20230120 Latest Revision: 20230120

20231215

10.1007/s00436-022-07761-8

36539638