Multivalent interactions facilitate motor-dependent protein accumulation at growing microtubule plus-ends.

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  • Additional Information
    • Source:
      Publisher: Macmillan Magazines Ltd Country of Publication: England NLM ID: 100890575 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1476-4679 (Electronic) Linking ISSN: 14657392 NLM ISO Abbreviation: Nat Cell Biol Subsets: MEDLINE
    • Publication Information:
      Original Publication: London : Macmillan Magazines Ltd., [1999-
    • Subject Terms:
      Microtubules*/metabolism ; Schizosaccharomyces*/genetics ; Schizosaccharomyces pombe Proteins*/genetics ; Schizosaccharomyces pombe Proteins*/metabolism; Dyneins/metabolism ; Kinesins/genetics ; Kinesins/metabolism ; Microtubule-Associated Proteins/genetics ; Microtubule-Associated Proteins/metabolism ; Myosins/metabolism
    • Abstract:
      Growing microtubule ends organize end-tracking proteins into comets of mixed composition. Here using a reconstituted fission yeast system consisting of end-binding protein Mal3, kinesin Tea2 and cargo Tip1, we found that these proteins can be driven into liquid-phase droplets both in solution and at microtubule ends under crowding conditions. In the absence of crowding agents, cryo-electron tomography revealed that motor-dependent comets consist of disordered networks where multivalent interactions may facilitate non-stoichiometric accumulation of cargo Tip1. We found that two disordered protein regions in Mal3 are required for the formation of droplets and motor-dependent accumulation of Tip1, while autonomous Mal3 comet formation requires only one of them. Using theoretical modelling, we explore possible mechanisms by which motor activity and multivalent interactions may lead to the observed enrichment of Tip1 at microtubule ends. We conclude that microtubule ends may act as platforms where multivalent interactions condense microtubule-associated proteins into large multi-protein complexes.
      (© 2022. The Author(s).)
    • Comments:
      Erratum in: Nat Cell Biol. 2023 Apr;25(4):626. doi: 10.1038/s41556-023-01124-w. (PMID: 36922647)
    • References:
      Nat Cell Biol. 2016 Oct;18(10):1102-8. (PMID: 27617931)
      Structure. 2018 Apr 3;26(4):607-618.e4. (PMID: 29576319)
      EMBO Rep. 2018 Nov;19(11):. (PMID: 30206188)
      Cell. 2019 Jan 24;176(3):419-434. (PMID: 30682370)
      Trends Cell Biol. 2019 Aug;29(8):612-622. (PMID: 31076235)
      EMBO J. 2022 Mar 1;41(5):e109952. (PMID: 35107832)
      Trends Biochem Sci. 2008 Nov;33(11):535-45. (PMID: 18835717)
      Phys Rev E. 2019 Aug;100(2-1):022145. (PMID: 31574637)
      EMBO J. 2005 Jan 26;24(2):261-9. (PMID: 15616574)
      Phys Rev Lett. 2003 Feb 28;90(8):086601. (PMID: 12633448)
      Cell. 2009 Jul 23;138(2):366-76. (PMID: 19632184)
      J Biol Chem. 2019 Jan 18;294(3):918-931. (PMID: 30455356)
      J Comput Chem. 2004 Oct;25(13):1605-12. (PMID: 15264254)
      Nat Cell Biol. 2023 Jan;25(1):56-67. (PMID: 36536177)
      Mol Biol Cell. 2011 Aug 15;22(16):2912-23. (PMID: 21737692)
      J Cell Biol. 2018 Aug 6;217(8):2691-2708. (PMID: 29794031)
      Curr Biol. 2004 Apr 6;14(7):548-59. (PMID: 15062095)
      J Cell Biol. 2002 May 27;157(5):783-93. (PMID: 12034771)
      Interface Focus. 2014 Dec 6;4(6):20140031. (PMID: 25485082)
      Elife. 2019 Sep 03;8:. (PMID: 31478831)
      Nat Rev Mol Cell Biol. 2017 May;18(5):285-298. (PMID: 28225081)
      Nature. 2003 Jun 5;423(6940):647-51. (PMID: 12789340)
      Nat Cell Biol. 2019 Sep;21(9):1078-1085. (PMID: 31481790)
      Nat Commun. 2013;4:1834. (PMID: 23673619)
      Proc Natl Acad Sci U S A. 2023 Aug 29;120(35):e2301457120. (PMID: 37603768)
      Curr Biol. 2014 Feb 17;24(4):372-84. (PMID: 24508171)
      Phys Rev Lett. 2012 Jun 22;108(25):258104. (PMID: 23004665)
      Nat Commun. 2020 Jan 14;11(1):270. (PMID: 31937751)
      Phys Rev E Stat Nonlin Soft Matter Phys. 2004 Oct;70(4 Pt 2):046101. (PMID: 15600454)
      Phys Rev Lett. 2001 Sep 3;87(10):108101. (PMID: 11531504)
      Science. 2009 Jun 26;324(5935):1729-32. (PMID: 19460965)
      Nat Phys. 2021 Apr;17(4):493-498. (PMID: 35211183)
      Nat Cell Biol. 2003 Sep;5(9):812-8. (PMID: 12894167)
      Proc Natl Acad Sci U S A. 2012 Apr 17;109(16):6100-5. (PMID: 22431622)
      J Cell Biol. 2004 Jun 7;165(5):697-707. (PMID: 15184402)
      J Cell Biol. 2005 Feb 14;168(4):587-98. (PMID: 15699215)
      J Cell Biol. 2022 Feb 7;221(2):. (PMID: 34878519)
      Nat Commun. 2017 Dec 13;8(1):2110. (PMID: 29235477)
      Nature. 2007 Dec 13;450(7172):1100-5. (PMID: 18059460)
      Annu Rev Cell Dev Biol. 2014;30:39-58. (PMID: 25288112)
      Cell. 2018 Jul 26;174(3):688-699.e16. (PMID: 29961577)
      Nat Struct Mol Biol. 2007 Oct;14(10):980-1. (PMID: 17828275)
      Nat Cell Biol. 2023 Jan;25(1):79-91. (PMID: 36536176)
      J Struct Biol. 2005 Oct;152(1):36-51. (PMID: 16182563)
      Dev Cell. 2004 Jun;6(6):831-43. (PMID: 15177031)
      Nat Cell Biol. 2019 Sep;21(9):1086-1092. (PMID: 31481789)
      Nat Methods. 2017 Apr;14(4):331-332. (PMID: 28250466)
      J Biol Chem. 2005 Apr 1;280(13):12299-304. (PMID: 15665379)
      J Cell Biol. 2008 Dec 29;183(7):1223-33. (PMID: 19103809)
      Cell Rep. 2017 Sep 5;20(10):2304-2312. (PMID: 28877466)
      J Cell Sci. 2016 Dec 15;129(24):4592-4606. (PMID: 27872152)
      Elife. 2019 Aug 13;8:. (PMID: 31405451)
      Science. 2015 May 15;348(6236):808-12. (PMID: 25977552)
      J Struct Biol. 1996 Jan-Feb;116(1):71-6. (PMID: 8742726)
      Elife. 2018 Apr 09;7:. (PMID: 29629870)
      Bioinformatics. 2015 Mar 15;31(6):857-63. (PMID: 25391399)
      Structure. 1996 Sep 15;4(9):1031-40. (PMID: 8805589)
      Nat Methods. 2017 Oct;14(10):983-985. (PMID: 28846087)
      EMBO J. 2005 Nov 2;24(21):3690-9. (PMID: 16222337)
      Cell. 2000 Sep 1;102(5):695-704. (PMID: 11007487)
      Biophys J. 2011 Nov 2;101(9):2190-200. (PMID: 22067158)
    • Accession Number:
      EC 3.6.4.2 (Dyneins)
      EC 3.6.4.4 (Kinesins)
      0 (Mal3 protein, S pombe)
      0 (Microtubule-Associated Proteins)
      EC 3.6.4.1 (Myosins)
      0 (Schizosaccharomyces pombe Proteins)
      0 (Tea2 protein, S pombe)
    • Publication Date:
      Date Created: 20221219 Date Completed: 20230223 Latest Revision: 20240914
    • Publication Date:
      20240914
    • Accession Number:
      PMC9859754
    • Accession Number:
      10.1038/s41556-022-01037-0
    • Accession Number:
      36536175