Visualizing Activated Myofibroblasts Resulting from Differentiation of Mesenchymal Stem Cells.

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  • Author(s): Mishra PJ;Mishra PJ; Banerjee D; Banerjee D
  • Source:
    Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2023; Vol. 2593, pp. 83-92.
  • Publication Type:
    Journal Article
  • Language:
    English
  • Additional Information
    • Source:
      Publisher: Humana Press Country of Publication: United States NLM ID: 9214969 Publication Model: Print Cited Medium: Internet ISSN: 1940-6029 (Electronic) Linking ISSN: 10643745 NLM ISO Abbreviation: Methods Mol Biol Subsets: MEDLINE
    • Publication Information:
      Publication: Totowa, NJ : Humana Press
      Original Publication: Clifton, N.J. : Humana Press,
    • Subject Terms:
      Myofibroblasts* ; Mesenchymal Stem Cells*; Humans ; Culture Media, Conditioned/pharmacology ; Culture Media, Conditioned/metabolism ; Cell Differentiation ; Keratinocytes/metabolism ; Transforming Growth Factor beta1/pharmacology
    • Abstract:
      Mesenchymal stem cells (MSCs) are multipotent cells that exhibit two main characteristics which define stem cells: self-renewal and differentiation. MSCs can migrate to sites of injury, inflammation, and tumor. Moreover, MSCs undergo myofibroblast-like differentiation, including increased production of α-SMA in response to transforming growth factor-β (TGF-β), a growth factor commonly secreted by tumor cells to evade immune surveillance. Based on our previous findings, hMSCs become activated and resemble carcinoma-associated myofibroblasts upon prolonged exposure to a conditioned medium from MDAMB231 human breast cancer cells. In this section, we show using immunofluorescence that keratinocyte-conditioned medium (KCM) induces differentiation of MSCs to resemble dermal myofibroblast-like cells with punctate vinculin staining and F-actin filaments.
      (© 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.)
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    • Contributed Indexing:
      Keywords: Breast cancer; Cell differentiation; Colorectal cancer; Dermal myofibroblast; Immunofluorescence; Mesenchymal stem cells; Migration; Myofibroblast; SDF-1; Stem cells
    • Accession Number:
      0 (Culture Media, Conditioned)
      0 (Transforming Growth Factor beta1)
    • Publication Date:
      Date Created: 20221213 Date Completed: 20221215 Latest Revision: 20230304
    • Publication Date:
      20230305
    • Accession Number:
      10.1007/978-1-0716-2811-9_5
    • Accession Number:
      36513925