Losartan Inhibition of Myofibroblast Generation and Late Haze (Scarring Fibrosis) After PRK in Rabbits.

Item request has been placed! ×
Item request cannot be made. ×
loading   Processing Request
Read More Add to Saved list
  • Author(s): Sampaio LP; Hilgert GSL; Shiju TM; Santhiago MR; Wilson SE
  • Source:
    Journal of refractive surgery (Thorofare, N.J. : 1995) [J Refract Surg] 2022 Dec; Vol. 38 (12), pp. 820-829. Date of Electronic Publication: 2022 Dec 01.
  • Publication Type:
    Journal Article
  • Language:
    English
  • Additional Information
    • Source:
      Publisher: SLACK Inc Country of Publication: United States NLM ID: 9505927 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1938-2391 (Electronic) Linking ISSN: 1081597X NLM ISO Abbreviation: J Refract Surg Subsets: MEDLINE
    • Publication Information:
      Original Publication: Thorofare, NJ : SLACK Inc., c1995-
    • Subject Terms:
      Losartan* ; Collagen Type IV*; United States ; Animals ; Rabbits ; Fibrosis ; Transforming Growth Factor beta
    • Abstract:
      Purpose: To study the effect of topical losartan compared to vehicle on the generation of myofibroblasts and development of late haze scarring fibrosis after photorefractive keratectomy (PRK) in rabbits.
      Methods: Twelve rabbits had -9.00 diopter (D) PRK in one eye followed by 50 µL of topical 0.2 mg/mL losartan or 50 µL of vehicle six times per day for 1 month. Standardized slit-lamp photographs were obtained prior to death. Duplex immunohistochemistry was performed on cryofixed corneas for myofibroblast marker alpha-smooth muscle actin (α-SMA) and keratocyte marker keratocan or collagen type IV and transforming growth factor (TGF)-β1. ImageJ software (National Institutes of Health) was used for quantitation.
      Results: Topical losartan compared to vehicle significantly decreased corneal opacity ( P = .04) and anterior stromal myofibroblast generation ( P = .01) at 1 month after PRK. Topical losartan compared to vehicle also decreased anterior stromal non-basement membrane collagen type IV at 1 month after PRK ( P = .004).
      Conclusions: Topical angiotensin converting enzyme II receptor inhibitor losartan, a known inhibitor of TGF-β signaling, decreased late haze scarring fibrosis and myofibroblast generation after -9.00 D PRK in rabbits compared to vehicle. It also decreases TGF-β-modulated, corneal fibroblast-produced, non-basement membrane stromal collagen type IV-likely also through inhibition of TGF-β signaling. [ J Refract Surg . 2022;38(12):820-829.] .
    • Accession Number:
      JMS50MPO89 (Losartan)
      0 (Collagen Type IV)
      0 (Transforming Growth Factor beta)
    • Publication Date:
      Date Created: 20221208 Date Completed: 20221215 Latest Revision: 20221215
    • Publication Date:
      20240829
    • Accession Number:
      10.3928/1081597X-20221026-03
    • Accession Number:
      36476304