Mitigation of atrazine-induced oxidative stress on soybean seedlings after co-inoculation with atrazine-degrading bacterium Arthrobacter sp. DNS10 and inorganic phosphorus-solubilizing bacterium Enterobacter sp. P1.

Publisher: Springer Country of Publication: Germany NLM ID: 9441769 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1614-7499 (Electronic) Linking ISSN: 09441344 NLM ISO Abbreviation: Environ Sci Pollut Res Int Subsets: MEDLINE

Publication: <2013->: Berlin : Springer
Original Publication: Landsberg, Germany : Ecomed

Atrazine*/analysis ; Herbicides*/analysis ; Arthrobacter*/metabolism; Glycine max/metabolism ; Seedlings/metabolism ; Enterobacter ; Chlorophyll A/analysis ; Biodegradation, Environmental ; Soil ; Oxidative Stress ; Antioxidants/metabolism ; Phosphorus/metabolism ; Soil Microbiology

Atrazine toxicity is one of the limiting factors inhibiting sensitive plant growth. Previous studies showed that atrazine-degrading bacteria could alleviate atrazine toxicity. However, there is limited information on how atrazine-degrading bacteria and plant growth-promote bacteria alleviate atrazine toxicity in soybeans. Therefore, the current study aimed to explore the atrazine removal, phosphorus utilization, and the oxidative stress alleviation of atrazine-degrading bacterium Arthrobacter sp. DNS10 and/or inorganic phosphorus-solubilizing bacterium Enterobacter sp. P1 in the reduction of atrazine toxicity in soybean. The results showed that atrazine exposure to soybean seedlings led to significant inhibition in growth, atrazine removal, and phosphorus utilization. However, the co-inoculatied strains significantly increased seedlings biomass, chlorophyll a/b contents, and total phosphorus in leaves accompanied by great reduction of the atrazine-induced antioxidant enzymes activities and malonaldehyde (MDA) contents, as well as atrazine contents in soil and soybeans under atrazine stress. Furthermore, transcriptome analysis highlighted that co-inoculated strains increased the expression levels of genes related to photosynthetic-antenna proteins, carbohydrate metabolism, and fatty acid degradation in leaves. All the results suggest that the co-inoculation mitigates atrazine-induced oxidative stress on soybean by accelerating atrazine removal from soil and phosphorus accumulation in leaves, enhancing the chlorophyll contents, and regulating plant transcriptome. It may be suggested that co-inoculation of atrazine-degrading bacteria and inorganic phosphorus-solubilizing bacteria can be used as a potential method to alleviate atrazine toxicity to the sensitive crops.
(© 2022. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)

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31972941 National Natural Science Foundation of China; U21A20238 Funds of the National Science Foundation of China; XDA28070302 Remediation, Strategic Priority Research Program of Chinese Academy of Sciences; YQ2021C010 Natural Science Foundation of Heilongjiang; CARS-04-PS21 Scientists of soybean industry technology system

Keywords: Alleviation; Atrazine; Bacterium; Degradation; Transcriptome

QJA9M5H4IM (Atrazine)
0 (Herbicides)
YF5Q9EJC8Y (Chlorophyll A)
0 (Soil)
0 (Antioxidants)
27YLU75U4W (Phosphorus)

Date Created: 20221123 Date Completed: 20230310 Latest Revision: 20231213

20231215

10.1007/s11356-022-24070-w

36418831