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Immobilization and Application of the Recombinant Xylanase GH10 of Malbranchea pulchella in the Production of Xylooligosaccharides from Hydrothermal Liquor of the Eucalyptus ( Eucalyptus grandis ) Wood Chips.
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- Author(s): Alnoch RC;Alnoch RC;Alnoch RC; Alves GS; Alves GS; Salgado JCS; Salgado JCS; de Andrades D; de Andrades D; Freitas EN; Freitas EN; Nogueira KMV; Nogueira KMV; Vici AC; Vici AC; Oliveira DP; Oliveira DP; Carvalho-Jr VP; Carvalho-Jr VP; Silva RN; Silva RN; Buckeridge MS; Buckeridge MS; Michelin M; Michelin M; Michelin M; Teixeira JA; Teixeira JA; Polizeli MLTM; Polizeli MLTM; Polizeli MLTM
- Source:
International journal of molecular sciences [Int J Mol Sci] 2022 Nov 01; Vol. 23 (21). Date of Electronic Publication: 2022 Nov 01.- Publication Type:
Journal Article- Language:
English - Source:
- Additional Information
- Source: Publisher: MDPI Country of Publication: Switzerland NLM ID: 101092791 Publication Model: Electronic Cited Medium: Internet ISSN: 1422-0067 (Electronic) Linking ISSN: 14220067 NLM ISO Abbreviation: Int J Mol Sci Subsets: MEDLINE
- Publication Information: Original Publication: Basel, Switzerland : MDPI, [2000-
- Subject Terms:
- Abstract: Xylooligosaccharides (XOS) are widely used in the food industry as prebiotic components. XOS with high purity are required for practical prebiotic function and other biological benefits, such as antioxidant and inflammatory properties. In this work, we immobilized the recombinant endo-1,4-β-xylanase of Malbranchea pulchella (MpXyn10) in various chemical supports and evaluated its potential to produce xylooligosaccharides (XOS) from hydrothermal liquor of eucalyptus wood chips. Values >90% of immobilization yields were achieved from amino-activated supports for 120 min. The highest recovery values were found on Purolite (142%) and MANAE-MpXyn10 (137%) derivatives, which maintained more than 90% residual activity for 24 h at 70 °C, while the free-MpXyn10 maintained only 11%. In addition, active MpXyn10 derivatives were stable in the range of pH 4.0−6.0 and the presence of the furfural and HMF compounds. MpXyn10 derivatives were tested to produce XOS from xylan of various sources. Maximum values were observed for birchwood xylan at 8.6 mg mL−1 and wheat arabinoxylan at 8.9 mg mL−1, using Purolite-MpXyn10. Its derivative was also successfully applied in the hydrolysis of soluble xylan present in hydrothermal liquor, with 0.9 mg mL−1 of XOS after 3 h at 50 °C. This derivative maintained more than 80% XOS yield after six cycles of the assay. The results obtained provide a basis for the application of immobilized MpXyn10 to produce XOS with high purity and other high-value-added products in the lignocellulosic biorefinery field.
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J Biotechnol. 2005 Sep 22;119(1):70-5. (PMID: 16039744) - Grant Information: 2014/50884-5 São Paulo Research Foundation; 2018/07522-6 São Paulo Research Foundation; 2020/15510-8 São Paulo Research Foundation; 2020/00081-4 São Paulo Research Foundation; FCT-POCI-01-0145-FEDER-032206 Fundação para a Ciência e Tecnologia; financial code 001 Coordenação de Aperfeicoamento de Pessoal de Nível Superior; 2019/21989-7 São Paulo Research Foundation; 465319/2014-9 National Council for Scientific and Technological Development
- Contributed Indexing: Keywords: Eucalyptus grandis; Malbranchea pulchella; hydrothermal liquor; immobilization; xylanases; xylooligosaccharides
- Accession Number: 0 (xylooligosaccharide)
0 (Xylans)
0 (Glucuronates)
0 (Oligosaccharides)
EC 3.2.1.8 (Endo-1,4-beta Xylanases)
0 (Prebiotics) - Subject Terms: Malbranchea pulchella
- Publication Date: Date Created: 20221111 Date Completed: 20221114 Latest Revision: 20230308
- Publication Date: 20231215
- Accession Number: PMC9656307
- Accession Number: 10.3390/ijms232113329
- Accession Number: 36362138
- Source:
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