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Lung Inflammasome Activation in SARS-CoV-2 Post-Mortem Biopsies.
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- Author(s): Baena Carstens L;Baena Carstens L; Campos D'amico R; Campos D'amico R; Campos D'amico R; Fernandes de Moura K; Fernandes de Moura K; Fernandes de Moura K; Morais de Castro E; Morais de Castro E; Centenaro F; Centenaro F; Silva Barbosa G; Silva Barbosa G; Vieira Cavalcante da Silva G; Vieira Cavalcante da Silva G; Brenny I; Brenny I; Honório D'Agostini JC; Honório D'Agostini JC; Hlatchuk EC; Hlatchuk EC; Pissette de Lima S; Pissette de Lima S; Camargo Martins AP; Camargo Martins AP; De Castro Deus M; De Castro Deus M; Konzen Klein C; Konzen Klein C; Kubaski Benevides AP; Kubaski Benevides AP; Nagashima S; Nagashima S; Nagashima S; Machado-Souza C; Machado-Souza C; Pinho RA; Pinho RA; Pellegrino Baena C; Pellegrino Baena C; Pellegrino Baena C; de Noronha L; de Noronha L; de Noronha L
- Source:
International journal of molecular sciences [Int J Mol Sci] 2022 Oct 27; Vol. 23 (21). Date of Electronic Publication: 2022 Oct 27.- Publication Type:
Journal Article- Language:
English - Source:
- Additional Information
- Source: Publisher: MDPI Country of Publication: Switzerland NLM ID: 101092791 Publication Model: Electronic Cited Medium: Internet ISSN: 1422-0067 (Electronic) Linking ISSN: 14220067 NLM ISO Abbreviation: Int J Mol Sci Subsets: MEDLINE
- Publication Information: Original Publication: Basel, Switzerland : MDPI, [2000-
- Subject Terms: COVID-19* ; Influenza A Virus, H1N1 Subtype*/metabolism; Humans ; Inflammasomes/metabolism ; SARS-CoV-2 ; Interleukin-18 ; NF-kappa B/metabolism ; Angiotensin-Converting Enzyme 2 ; Autopsy ; Caspase 1/metabolism ; Lung/metabolism ; Cytokines/metabolism ; Biopsy ; NLR Family, Pyrin Domain-Containing 3 Protein/metabolism
- Abstract: The inflammasome complex is a key part of chronic diseases and acute infections, being responsible for cytokine release and cell death mechanism regulation. The SARS-CoV-2 infection is characterized by a dysregulated cytokine release. In this context, the inflammasome complex analysis within SARS-CoV-2 infection may prove beneficial to understand the disease’s mechanisms. Post-mortem minimally invasive autopsies were performed in patients who died from COVID-19 (n = 24), and lung samples were compared to a patient control group (n = 11) and an Influenza A virus H1N1 subtype group from the 2009 pandemics (n = 10). Histological analysis was performed using hematoxylin-eosin staining. Immunohistochemical (IHC) staining was performed using monoclonal antibodies against targets: ACE2, TLR4, NF-κB, NLRP-3 (or NALP), IL-1β, IL-18, ASC, CASP1, CASP9, GSDMD, NOX4, TNF-α. Data obtained from digital analysis underwent appropriate statistical tests. IHC analysis showed biomarkers that indicate inflammasome activation (ACE2; NF-κB; NOX4; ASC) were significantly increased in the COVID-19 group (p < 0.05 for all) and biomarkers that indicate cell pyroptosis and inflammasome derived cytokines such as IL-18 (p < 0.005) and CASP1 were greatly increased (p < 0.0001) even when compared to the H1N1 group. We propose that the SARS-CoV-2 pathogenesis is connected to the inflammasome complex activation. Further studies are still warranted to elucidate the pathophysiology of the disease.
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- Contributed Indexing: Keywords: COVID-19; cytokine; immunohistochemistry; inflammasome; oxidative stress; pulmonary tissue; pyroptosis
- Accession Number: 0 (Inflammasomes)
0 (Interleukin-18)
0 (NF-kappa B)
EC 3.4.17.23 (Angiotensin-Converting Enzyme 2)
EC 3.4.22.36 (Caspase 1)
0 (Cytokines)
0 (NLR Family, Pyrin Domain-Containing 3 Protein) - Publication Date: Date Created: 20221111 Date Completed: 20221114 Latest Revision: 20230308
- Publication Date: 20231215
- Accession Number: PMC9659061
- Accession Number: 10.3390/ijms232113033
- Accession Number: 36361818
- Source:
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