Monocyte-derived peritoneal macrophages protect C57BL/6 mice against surgery-induced adhesions.

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  • Additional Information
    • Source:
      Publisher: Frontiers Research Foundation] Country of Publication: Switzerland NLM ID: 101560960 Publication Model: eCollection Cited Medium: Internet ISSN: 1664-3224 (Electronic) Linking ISSN: 16643224 NLM ISO Abbreviation: Front Immunol Subsets: MEDLINE
    • Publication Information:
      Original Publication: [Lausanne : Frontiers Research Foundation]
    • Subject Terms:
      Monocytes*/pathology ; Macrophages, Peritoneal*; Mice ; Female ; Animals ; Mice, Inbred C57BL ; Cicatrix/pathology ; Macrophages/pathology ; Tissue Adhesions ; Receptors, Chemokine ; Mice, Inbred BALB C
    • Abstract:
      Peritoneal adhesions commonly occur after abdominal or pelvic surgery. These scars join internal organs to each other or to the cavity wall and can present with abdominal or pelvic pain, and bowel obstruction or female infertility. The mechanisms underlying adhesion formation remain unclear and thus, effective treatments are not forthcoming. Peritoneal macrophages accumulate after surgery and previous studies have attributed either pro- or anti-scarring properties to these cells. We propose that there are complex and nuanced responses after surgery with respect to both resident and also monocyte-derived peritoneal macrophage subpopulations. Moreover, we contend that differences in responses of specific macrophage subpopulations in part explain the risk of developing peritoneal scars. We characterized alterations in peritoneal macrophage subpopulations after surgery-induced injury using two strains of mice, BALB/c and C57BL/6, with known differences in macrophage response post-infection. At 14 days post-surgery, BALB/c mice displayed more adhesions compared with C57BL/6 mice. This increase in scarring correlated with a lower influx of monocyte-derived macrophages at day 3 post-surgery. Moreover, BALB/c mice showed distinct macrophage repopulation dynamics after surgery. To confirm a role for monocyte-derived macrophages, we used Ccr2 -deficient mice as well as antibody-mediated depletion of CCR2 expressing cells during initial stages of adhesion formation. Both Ccr2 -deficient and CCR2-depleted mice showed a significant increase in adhesion formation associated with the loss of peritoneal monocyte influx. These findings revealed an important protective role for monocyte-derived cells in reducing adhesion formation after surgery.
      Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
      (Copyright © 2022 Sahputra, Dejyong, Woolf, Mack, Allen, Rückerl and Herrick.)
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    • Grant Information:
      MR/P02615X/1 United Kingdom MRC_ Medical Research Council; MR/S02560X/1 United Kingdom MRC_ Medical Research Council
    • Contributed Indexing:
      Keywords: adhesion formation; macrophages; monocytes; peritoneum; post-surgery
    • Accession Number:
      0 (Receptors, Chemokine)
    • Publication Date:
      Date Created: 20221024 Date Completed: 20221025 Latest Revision: 20240906
    • Publication Date:
      20240906
    • Accession Number:
      PMC9583908
    • Accession Number:
      10.3389/fimmu.2022.1000491
    • Accession Number:
      36275765