Discovery of Aptamers Against Cell Surface Markers Using Ligand-Guided Selection.

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  • Additional Information
    • Source:
      Publisher: Humana Press Country of Publication: United States NLM ID: 9214969 Publication Model: Print Cited Medium: Internet ISSN: 1940-6029 (Electronic) Linking ISSN: 10643745 NLM ISO Abbreviation: Methods Mol Biol Subsets: MEDLINE
    • Publication Information:
      Publication: Totowa, NJ : Humana Press
      Original Publication: Clifton, N.J. : Humana Press,
    • Subject Terms:
      Aptamers, Nucleotide*/genetics ; Aptamers, Nucleotide*/metabolism ; SELEX Aptamer Technique*/methods; Antibodies, Monoclonal ; Humans ; Ligands ; RNA ; Receptors, Antigen, T-Cell
    • Abstract:
      Oligonucleotide ligands (DNA, RNA, or XNA), also known as aptamers, are selected against various target molecules using an iterative, evolutionary process called systematic evolution of ligands by exponential enrichment (SELEX). To select aptamers against complex cell surface proteins in their native state, a variant of SELEX termed ligand-guided selection (LIGS) was recently introduced. The significance of LIGS is rooted in its strategy of exploiting the selection step in SELEX to identify highly specific aptamers against known cell surface markers. Thus, in LIGS, a higher-affinity secondary ligand, such as a monoclonal antibody (mAb) to a whole-cell bound to an evolved SELEX library, is introduced to outcompete sequences against the mAb targeting cell surface protein or induce a conformational switch to destabilize the aptamer-surface cell surface protein resulting in elution of the sequences. Here, we describe the detailed method of LIGS utilized in identifying aptamers against T-cell receptor cluster of differentiation three complex (TCR-CD3) expressed in human T-cells and T-cell leukemia.
      (© 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.)
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    • Grant Information:
      R35 GM139336 United States GM NIGMS NIH HHS; SC1 GM122648 United States GM NIGMS NIH HHS
    • Contributed Indexing:
      Keywords: Cell-SELEX; Enriched DNA library; LIGS; NGS sequencing; Oligonucleotide ligands; SELEX; TCR-CD3; Target-specific aptamer
    • Accession Number:
      0 (Antibodies, Monoclonal)
      0 (Aptamers, Nucleotide)
      0 (Ligands)
      0 (Receptors, Antigen, T-Cell)
      63231-63-0 (RNA)
    • Publication Date:
      Date Created: 20220926 Date Completed: 20220928 Latest Revision: 20230321
    • Publication Date:
      20240829
    • Accession Number:
      10.1007/978-1-0716-2695-5_2
    • Accession Number:
      36156771