Quantitation of Fatty Acids in Serum/Plasma and Red Blood Cells by Gas Chromatography-Negative Chemical Ionization-Mass Spectrometry.

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  • Additional Information
    • Source:
      Publisher: Humana Press Country of Publication: United States NLM ID: 9214969 Publication Model: Print Cited Medium: Internet ISSN: 1940-6029 (Electronic) Linking ISSN: 10643745 NLM ISO Abbreviation: Methods Mol Biol Subsets: MEDLINE
    • Publication Information:
      Publication: Totowa, NJ : Humana Press
      Original Publication: Clifton, N.J. : Humana Press,
    • Subject Terms:
      Erythrocytes* ; Fatty Acids, Essential*; Erythrocyte Count ; Gas Chromatography-Mass Spectrometry/methods ; Humans
    • Abstract:
      Quantitation of long-chain fatty acids in serum/plasma and red blood cells is a useful diagnostic tool in the evaluation of nutritional status and assessment of risk for essential fatty acid deficiency (EFAD). Serum/plasma has been the traditional sample type for this method, yet it requires prolonged fasting which is not compatible with some patient populations. More recently, red blood cells have become an important sample type due to less intraindividual variability and obviating the need for fasting. Here we present a method for the quantitation of 22 fatty acids in serum/plasma or red blood cells. Fatty acids are hydrolyzed and extracted from the biological matrix, followed by derivatization with pentafluorobenzyl bromide and subsequent analysis by gas chromatography-negative chemical ionization-mass spectrometry (GC-NCI-MS).
      (© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.)
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    • Contributed Indexing:
      Keywords: Chemical ionization; Essential fatty acid deficiency; Fatty acids; Gas chromatography; Mass spectrometry; Plasma; Red blood cells; Selected ion monitoring; Serum
    • Accession Number:
      0 (Fatty Acids, Essential)
    • Publication Date:
      Date Created: 20220920 Date Completed: 20220923 Latest Revision: 20230214
    • Publication Date:
      20240829
    • Accession Number:
      10.1007/978-1-0716-2565-1_14
    • Accession Number:
      36127586