IL-22 regulates endometrial regeneration by enhancing tight junctions and orchestrating extracellular matrix.

Publisher: Frontiers Research Foundation] Country of Publication: Switzerland NLM ID: 101560960 Publication Model: eCollection Cited Medium: Internet ISSN: 1664-3224 (Electronic) Linking ISSN: 16643224 NLM ISO Abbreviation: Front Immunol Subsets: MEDLINE

Original Publication: [Lausanne : Frontiers Research Foundation]

Endometrium*/immunology ; Extracellular Matrix*/genetics ; Extracellular Matrix*/immunology ; Inflammation*/genetics ; Inflammation*/immunology ; Interleukins*/genetics ; Interleukins*/immunology ; Regeneration*/immunology ; Tight Junctions*/immunology; Abortion, Spontaneous/immunology ; Animals ; Female ; Humans ; Infertility/genetics ; Infertility/immunology ; Lipopolysaccharides/immunology ; Mice ; Pregnancy ; Interleukin-22

The uterine endometrium uniquely regenerates after menses, postpartum, or after breaks in the uterine layer integrity throughout women's lives. Direct cell-cell contacts ensured by tight and adherens junctions play an important role in endometrial integrity. Any changes in these junctions can alter the endometrial permeability of the uterus and have an impact on the regeneration of uterine layers. Interleukin 22 (IL-22) is a cytokine that is recognized for its role in epithelial regeneration. Moreover, it is crucial in controlling the inflammatory response in mucosal tissues. Here, we studied the role of IL-22 in endometrial recovery after inflammation-triggered abortion. Fecundity of mice was studied in consecutive matings of the same animals after lipopolysaccharide (LPS) (10 µg per mouse)-triggered abortion. The fecundity rate after the second mating was substantially different between IL-22 knockout (IL-22 ^-/- ) (9.1%) and wild-type (WT) (71.4%) mice (p < 0.05), while there was no difference between the groups in the initial mating, suggesting that IL-22 deficiency might be associated with secondary infertility. A considerable difference was observed between IL-22 ^-/- and WT mice in the uterine clearance following LPS-triggered abortion. Gross examination of the uteri of IL-22 ^-/- mice revealed non-viable fetuses retained inside the horns (delayed clearance). In contrast, all WT mice had completed abortion with total clearance after LPS exposure. We also discovered that IL-22 deficiency is associated with a decreased expression of tight junctions (claudin-2 and claudin-10) and cell surface pathogen protectors (mucin-1). Moreover, IL-22 has a role in the remodeling of the uterine tissue in the inflammatory environment by regulating epithelial-mesenchymal transition markers called E- and N-cadherin. Therefore, IL-22 contributes to the proper regeneration of endometrial layers after inflammation-triggered abortion. Thus, it might have a practical significance to be utilized as a treatment option postpartum (enhanced regeneration function) and in secondary infertility caused by inflammation (enhanced barrier/protector function).
Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
(Copyright © 2022 Ganieva, Schneiderman, Bu, Beaman and Dambaeva.)

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Keywords: IL-22; IL-22-/- mice; claudin-10; claudin-2; lipopolysaccharide; mucin-1; tight junctions; uterine regeneration

0 (Interleukins)
0 (Lipopolysaccharides)

Date Created: 20220912 Date Completed: 20220913 Latest Revision: 20231213

20231215

PMC9453595

10.3389/fimmu.2022.955576

36091010