TiO 2 nanoparticles added to dense bovine hydroxyapatite bioceramics increase human osteoblast mineralization activity.

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  • Additional Information
    • Source:
      Publisher: Elsevier Science Country of Publication: England NLM ID: 8508040 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-0097 (Electronic) Linking ISSN: 01095641 NLM ISO Abbreviation: Dent Mater
    • Publication Information:
      Publication: 1998- : Kidlington, Oxford, UK : Elsevier Science
      Original Publication: Copenhagen : Munksgaard, c1985-
    • Subject Terms:
      Durapatite*/chemistry ; Durapatite*/pharmacology ; Nanoparticles*; Animals ; Cattle ; Humans ; Microscopy, Electron, Scanning ; Osteoblasts ; Oxides/pharmacology ; Powders ; Surface Properties ; Titanium/chemistry ; Titanium/pharmacology
    • Abstract:
      Objectives: This study evaluated the effect of TiO 2 nanoparticles + dense hydroxyapatite (HA) on human osteoblast cells (SAOS-2).
      Methods: Particulate bovine HA powder with or without the addition of either 5 or 8 % TiO 2 (HA, HA/TiO 2 Np5 % or HA/TiO 2 Np8 %) were pressed into disks (Ø = 12.5 mm; thickness = 1.3 mm) uniaxially (100 MPa) and isostatically (200 MPa/1 min) and sintered at 1300 °C. Y-TZP disks were used as control. The following tests were performed: Scanning Electron Microscopy and Dispersive Energy Spectroscopy (SEM/EDS), Atomic Force Microscopy (AFM), cell viability assay (Alamar Blue-AB) and mineralized matrix deposition (Alizarin Red-AR). AB and AR data were submitted to 2-way ANOVA/Tukey tests and ANOVA/Tukey tests, respectively.
      Results: SEM revealed that the surface of HA/TiO 2 Np5% resembles DPBHA surface, but also contains smaller granules. HA/TiO 2 Np8% characteristics resembles HA/TiO 2 Np5% surface, but with irregular topography. Y-TZP showed a typical oxide ceramic surface pattern. EDS revealed Ca, O, and P in all samples. C, O, and Zr appeared in Y-TZP samples. AFM data corroborates SEM analysis. AB test revealed excellent cellular viability for HA/TiO 2 Np5% group. AR test showed that all groups containing TiO 2 np had more mineralized matrix deposition than all other groups, with statistically differences between HA/TiO 2 Np8% and HA cultivated in non-osteogenic medium. Culture in osteogenic medium exhibited much more mineralized matrix deposition by TiO 2 np groups.
      Significance: In conclusion, the addition of TiO 2 np showed chemical, superficial, and biological changes in the reinforced materials. HA/TiO 2 Np5% showed the best results for cell viability and HA/TiO 2 Np8% for mineralized matrix deposition.
      Competing Interests: Declaration of Competing Interest None.
      (Copyright © 2022 Elsevier Inc. All rights reserved.)
    • Contributed Indexing:
      Keywords: Biocompatible Materials; Ceramics; Hydroxyapatite; Titanium dioxide
    • Accession Number:
      0 (Oxides)
      0 (Powders)
      15FIX9V2JP (titanium dioxide)
      91D9GV0Z28 (Durapatite)
      D1JT611TNE (Titanium)
    • Publication Date:
      Date Created: 20220906 Date Completed: 20221019 Latest Revision: 20221226
    • Publication Date:
      20221227
    • Accession Number:
      10.1016/j.dental.2022.08.007
    • Accession Number:
      36068105