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Constitutive activation of a nuclear-localized calcium channel complex in Medicago truncatula .
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- Additional Information
- Source:
Publisher: National Academy of Sciences Country of Publication: United States NLM ID: 7505876 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1091-6490 (Electronic) Linking ISSN: 00278424 NLM ISO Abbreviation: Proc Natl Acad Sci U S A Subsets: MEDLINE
- Publication Information:
Original Publication: Washington, DC : National Academy of Sciences
- Subject Terms:
- Abstract:
Nuclear Ca 2+ oscillations allow symbiosis signaling, facilitating plant recognition of beneficial microsymbionts, nitrogen-fixing rhizobia, and nutrient-capturing arbuscular mycorrhizal fungi. Two classes of channels, DMI1 and CNGC15, in a complex on the nuclear membrane, coordinate symbiotic Ca 2+ oscillations. However, the mechanism of Ca 2+ signature generation is unknown. Here, we demonstrate spontaneous activation of this channel complex, through gain-of-function mutations in DMI1 , leading to spontaneous nuclear Ca 2+ oscillations and spontaneous nodulation, in a CNGC15 -dependent manner. The mutations destabilize a hydrogen-bond or salt-bridge network between two RCK domains, with the resultant structural changes, alongside DMI1 cation permeability, activating the channel complex. This channel complex was reconstituted in human HEK293T cell lines, with the resultant calcium influx enhanced by autoactivated DMI1 and CNGC15s. Our results demonstrate the mode of activation of this nuclear channel complex, show that DMI1 and CNGC15 are sufficient to create oscillatory Ca 2+ signals, and provide insights into its native mode of induction.
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- Contributed Indexing:
Keywords: CNGC15; DMI1; NF signaling; calcium channel; nuclear calcium spiking
- Accession Number:
0 (Calcium Channels)
0 (Plant Proteins)
SY7Q814VUP (Calcium)
- Publication Date:
Date Created: 20220816 Date Completed: 20220818 Latest Revision: 20230217
- Publication Date:
20240829
- Accession Number:
PMC9407390
- Accession Number:
10.1073/pnas.2205920119
- Accession Number:
35972963
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