Characterizing the genetic basis of trait evolution in the Mexican cavefish.

Publisher: Blackwell Science Country of Publication: United States NLM ID: 100883432 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1525-142X (Electronic) Linking ISSN: 1520541X NLM ISO Abbreviation: Evol Dev Subsets: MEDLINE

Original Publication: Malden, MA : Blackwell Science, c1999-

Biological Evolution* ; Characidae*/genetics; Adaptation, Physiological ; Animals ; Mexico ; Phenotype

Evolution in response to a change in ecology often coincides with various morphological, physiological, and behavioral traits. For most organisms little is known about the genetic and functional relationship between evolutionarily derived traits, representing a critical gap in our understanding of adaptation. The Mexican tetra, Astyanax mexicanus, consists of largely independent populations of fish that inhabit at least 30 caves in Northeast Mexico, and a surface fish population, that inhabit the rivers of Mexico and Southern Texas. The recent application of molecular genetic approaches combined with behavioral phenotyping have established A. mexicanus as a model for studying the evolution of complex traits. Cave populations of A. mexicanus are interfertile with surface populations and have evolved numerous traits including eye degeneration, insomnia, albinism, and enhanced mechanosensory function. The interfertility of different populations from the same species provides a unique opportunity to define the genetic relationship between evolved traits and assess the co-evolution of behavioral and morphological traits with one another. To define the relationships between morphological and behavioral traits, we developed a pipeline to test individual fish for multiple traits. This pipeline confirmed differences in locomotor activity, prey capture, and startle reflex between surface and cavefish populations. To measure the relationship between traits, individual F2 hybrid fish were characterized for locomotor behavior, prey-capture behavior, startle reflex, and morphological attributes. Analysis revealed an association between body length and slower escape reflex, suggesting a trade-off between increased size and predator avoidance in cavefish. Overall, there were few associations between individual behavioral traits, or behavioral and morphological traits, suggesting independent genetic changes underlie the evolution of the measured behavioral and morphological traits. Taken together, this approach provides a novel system to identify genetic underpinnings of naturally occurring variation in morphological and behavioral traits.
(© 2022 The Authors. Evolution & Development published by Wiley Periodicals LLC.)

Dev Biol. 2001 Mar 1;231(1):1-12. (PMID: 11180948)
J Exp Zool B Mol Dev Evol. 2020 Nov;334(7-8):423-437. (PMID: 32614138)
Mol Ecol. 2018 Nov;27(22):4397-4416. (PMID: 30252986)
CSH Protoc. 2008 Nov 01;2008:pdb.prot5093. (PMID: 21356733)
Dev Biol. 2018 Sep 15;441(2):313-318. (PMID: 29555241)
PLoS One. 2015 Mar 16;10(3):e0119370. (PMID: 25774757)
J Exp Biol. 2018 Jun 12;221(Pt 11):. (PMID: 29895581)
Nat Genet. 2006 Jan;38(1):107-11. (PMID: 16341223)
Nat Commun. 2021 Mar 4;12(1):1447. (PMID: 33664263)
Mol Phylogenet Evol. 2004 Nov;33(2):469-81. (PMID: 15336680)
J Exp Zool B Mol Dev Evol. 2020 Nov;334(7-8):474-485. (PMID: 32779370)
Dev Biol. 2018 Sep 15;441(2):235-241. (PMID: 30017604)
Curr Biol. 2013 Oct 7;23(19):1874-83. (PMID: 24035545)
Curr Biol. 2011 Apr 26;21(8):671-6. (PMID: 21474315)
Nat Commun. 2014 Oct 20;5:5307. (PMID: 25329095)
Philos Trans R Soc Lond B Biol Sci. 2017 Feb 5;372(1713):. (PMID: 27994127)
Evol Dev. 2008 Mar-Apr;10(2):196-209. (PMID: 18315813)
Sci Adv. 2020 Sep 16;6(38):. (PMID: 32938683)
Evodevo. 2020 Jul 11;11:14. (PMID: 32676179)
J Exp Biol. 2017 Jan 15;220(Pt 2):284-293. (PMID: 28100806)
Nat Methods. 2012 Jun 28;9(7):676-82. (PMID: 22743772)
Proc Biol Sci. 2018 May 16;285(1878):. (PMID: 29720416)
Annu Rev Genet. 2009;43:25-47. (PMID: 19640230)
Evol Dev. 2022 Sep;24(5):131-144. (PMID: 35924750)
Dev Dyn. 2009 Dec;238(12):3056-64. (PMID: 19877280)
J Exp Zool B Mol Dev Evol. 2020 Nov;334(7-8):397-404. (PMID: 32638529)
Evol Dev. 2008 May-Jun;10(3):265-72. (PMID: 18460088)
Front Neuroanat. 2019 Oct 04;13:88. (PMID: 31636546)
Dev Biol. 2016 Jul 15;415(2):338-346. (PMID: 26719128)
Zebrafish. 2011 Dec;8(4):155-65. (PMID: 22181659)
Genetics. 2014 Apr;196(4):1303-19. (PMID: 24496009)
Philos Trans R Soc Lond B Biol Sci. 2017 Feb 5;372(1713):. (PMID: 27994128)
Dev Biol. 2018 Sep 15;441(2):328-337. (PMID: 29772227)
Curr Biol. 2018 Jan 22;28(2):R60-R64. (PMID: 29374443)
Mol Reprod Dev. 2015 Apr;82(4):268-80. (PMID: 25728684)
BMC Evol Biol. 2012 Jun 30;12:105. (PMID: 22747496)
Nat Rev Genet. 2018 Nov;19(11):705-717. (PMID: 30111830)
J Vis Exp. 2019 Apr 22;(146):. (PMID: 31058898)
PLoS One. 2013 Nov 25;8(11):e80823. (PMID: 24282555)
Front Genet. 2017 Dec 07;8:205. (PMID: 29270192)
Evodevo. 2014 Oct 03;5:35. (PMID: 25908953)
J Exp Biol. 2014 Jun 1;217(Pt 11):1955-62. (PMID: 24675558)
Nat Rev Genet. 2004 Apr;5(4):288-98. (PMID: 15131652)
Zebrafish. 2008 Summer;5(2):97-110. (PMID: 18554173)
Curr Biol. 2021 Aug 23;31(16):3694-3701.e4. (PMID: 34293332)
Curr Biol. 2008 Jan 8;18(1):R23-4. (PMID: 18177707)
Nat Rev Genet. 2013 Nov;14(11):751-64. (PMID: 24105273)
J Exp Biol. 2020 Feb 7;223(Pt Suppl 1):. (PMID: 32034044)
BMC Biol. 2012 Dec 27;10:108. (PMID: 23270452)
Curr Biol. 2010 Sep 28;20(18):1631-6. (PMID: 20705469)
Sci Rep. 2019 Oct 31;9(1):15705. (PMID: 31673067)
J Evol Biol. 2011 Jan;24(1):206-18. (PMID: 21044205)
Development. 2011 Jun;138(12):2467-76. (PMID: 21610028)
Dev Biol. 2009 Jun 1;330(1):200-11. (PMID: 19285488)

R01 GM127872 United States GM NIGMS NIH HHS; T34 GM136486 United States GM NIGMS NIH HHS; 1923372 NSF

Keywords: behavior; cavefish; development

Date Created: 20220804 Date Completed: 20220923 Latest Revision: 20221229

20231215

PMC9786752

10.1111/ede.12412

35924750