Lef1 and Dlx3 May Facilitate the Maturation of Secondary Hair Follicles in the Skin of Gansu Alpine Merino.

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  • Additional Information
    • Source:
      Publisher: MDPI Country of Publication: Switzerland NLM ID: 101551097 Publication Model: Electronic Cited Medium: Internet ISSN: 2073-4425 (Electronic) Linking ISSN: 20734425 NLM ISO Abbreviation: Genes (Basel) Subsets: MEDLINE
    • Publication Information:
      Original Publication: Basel : MDPI
    • Subject Terms:
      Hair Follicle* ; Homeodomain Proteins*/genetics ; Lymphoid Enhancer-Binding Factor 1*/genetics ; Transcription Factors*/genetics; Animals ; Regulatory Sequences, Nucleic Acid ; Sheep, Domestic/genetics ; Skin/metabolism
    • Abstract:
      Lymphatic enhancer factor 1 (Lef1) and distal-less homeobox 3 (Dlx3) are the transcription factors involved in regulating hair follicle development in mice, goats, and other animals. Their deletion can lead to hair follicle deficiency. In this study, hematoxylin−eosin staining (HE), real-time quantitative PCR (RT-qPCR), immunohistochemistry, and immunofluorescence were used to analyze the expression, location, and biological functions of Lef1 and Dlx3 in the lateral skin of Gansu Alpine Merino aged 1, 30, 60, and 90 days. The results revealed that the number of hair follicles decreased with age and was significantly higher at 1 day than in the other three age groups (p < 0.05). The mRNA levels of Lef1 and Dlx3 in the skin of 30-day old Gansu Alpine Merino were significantly higher than those in the other three age groups (p < 0.05). Protein expression of Lef1 and Dlx3 was lowest at 1 day (p < 0.05) and peaked at 60 days. Lef1 and Dlx3 exhibited a high density and strong positive expression in the dermal papillae; additionally, Dlx3 exhibited a high density and strong positive expression in the inner and outer root sheaths. Collectively, Lef1 and Dlx3 may facilitate the maturation of secondary hair follicles, which is mainly achieved through the dermal papillae and inner and outer root sheaths.
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    • Contributed Indexing:
      Keywords: Dlx3; Gansu Alpine Merino; Lef1; hair follicle; spatiotemporal expression
    • Accession Number:
      0 (Homeodomain Proteins)
      0 (Lymphoid Enhancer-Binding Factor 1)
      0 (Transcription Factors)
    • Publication Date:
      Date Created: 20220727 Date Completed: 20220728 Latest Revision: 20230308
    • Publication Date:
      20240829
    • Accession Number:
      PMC9394301
    • Accession Number:
      10.3390/genes13081326
    • Accession Number:
      35893063