Expression of the nuclear encoded OEE1 protein is required for oxygen evolution and stability of photosystem II particles in Chlamydomonas reinhardtii.

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  • Author(s): Mayfield SP; Bennoun P; Rochaix JD
  • Source:
    The EMBO journal [EMBO J] 1987 Feb; Vol. 6 (2), pp. 313-8.
  • Publication Type:
    Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.
  • Language:
    English
  • Additional Information
    • Source:
      Publisher: Wiley Blackwell Country of Publication: England NLM ID: 8208664 Publication Model: Print Cited Medium: Print ISSN: 0261-4189 (Print) Linking ISSN: 02614189 NLM ISO Abbreviation: EMBO J Subsets: MEDLINE
    • Publication Information:
      Publication: 2014- : London : Wiley Blackwell
      Original Publication: Eynsham, Oxford, England : Published for the European Molecular Biology Organization by IRL Press, [c1982-
    • Subject Terms:
      Algal Proteins* ; Genes*; Chlamydomonas/*genetics ; Chlorophyll/*genetics ; Oxygen/*metabolism ; Plant Proteins/*genetics; Cell Nucleus/metabolism ; Chlamydomonas/metabolism ; Light-Harvesting Protein Complexes ; Mutation ; Photosynthetic Reaction Center Complex Proteins ; Photosystem II Protein Complex ; RNA, Messenger/genetics ; Transcription, Genetic
    • Abstract:
      In Chlamydomonas reinhardtii the oxygen evolving enhancer protein 1 (OEE1), which is part of the oxygen evolving complex of photosystem II (PS II), is coded for by a single nuclear gene (psb1). The nuclear mutant FuD44 specifically lacks the OEE1 polypeptide and is completely deficient in photosynthetic oxygen evolution. In this mutant a 5 kb DNA insertion into the 5' region of the psb1 gene results in the complete absence of OEE1 mRNA and protein. A revertant, FuD44-R 2, which is capable of 30% of the photosynthetic oxygen evolution of wild-type cells, has lost 4 kb of the 5 kb DNA insert, and accumulates both OEE1 mRNA and protein, although at levels somewhat less than those of wild-type cells. Absence of the OEE1 protein in the FuD44 mutant does not affect the accumulation of other nuclear encoded PS II peripheral polypeptides. OEE1 absence does, however, result in a more rapid turnover of the chloroplast encoded PS II core polypeptides, thus resulting in a substantial deficiency of PS II core polypeptides in FuD44 cells. These PS II core proteins again accumulate in revertant FuD44-R2 cells.
    • References:
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    • Grant Information:
      GM10246-02 United States GM NIGMS NIH HHS
    • Accession Number:
      0 (Algal Proteins)
      0 (Light-Harvesting Protein Complexes)
      0 (Photosynthetic Reaction Center Complex Proteins)
      0 (Photosystem II Protein Complex)
      0 (Plant Proteins)
      0 (RNA, Messenger)
      0 (oxygen-evolving enhancer protein 1, plant)
      1406-65-1 (Chlorophyll)
      S88TT14065 (Oxygen)
    • Publication Date:
      Date Created: 19870201 Date Completed: 19870706 Latest Revision: 20220511
    • Publication Date:
      20231215
    • Accession Number:
      PMC553397
    • Accession Number:
      10.1002/j.1460-2075.1987.tb04756.x
    • Accession Number:
      3556163