Bio-fabrication of stem-cell-incorporated corneal epithelial and stromal equivalents from silk fibroin and gelatin-based biomaterial for canine corneal regeneration.

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  • Additional Information
    • Source:
      Publisher: Public Library of Science Country of Publication: United States NLM ID: 101285081 Publication Model: eCollection Cited Medium: Internet ISSN: 1932-6203 (Electronic) Linking ISSN: 19326203 NLM ISO Abbreviation: PLoS One Subsets: MEDLINE
    • Publication Information:
      Original Publication: San Francisco, CA : Public Library of Science
    • Subject Terms:
      Regeneration*; Biocompatible Materials/*chemistry ; Epithelium, Corneal/*pathology ; Stem Cells/*cytology ; Stromal Cells/*cytology; 3T3 Cells ; ATP Binding Cassette Transporter, Subfamily G, Member 2/metabolism ; Animals ; Aquaporin 1/metabolism ; Cell Proliferation ; Collagen Type I/metabolism ; Corneal Transplantation ; Dogs ; Eye Proteins/metabolism ; Fibroblasts/cytology ; Fibroins/chemistry ; Gelatin/chemistry ; Genes, Tumor Suppressor ; Immunohistochemistry ; In Vitro Techniques ; Lumican/metabolism ; Mice ; Tensile Strength ; Tissue Engineering ; Tissue Scaffolds
    • Abstract:
      Corneal grafts are the imperative clinical treatment for canine corneal blindness. To serve the growing demand, this study aimed to generate tissue-engineered canine cornea in part of the corneal epithelium and underlying stroma based on canine limbal epithelial stem cells (cLESCs) seeded silk fibroin/gelatin (SF/G) film and canine corneal stromal stem cells (cCSSCs) seeded SF/G scaffold, respectively. Both cell types were successfully isolated by collagenase I. SF/G corneal films and stromal scaffolds served as the prospective substrates for cLESCs and cCSSCs by promoting cell adhesion, cell viability, and cell proliferation. The results revealed the upregulation of tumor protein P63 (P63) and ATP-binding cassette super-family G member 2 (Abcg2) of cLESCs as well as Keratocan (Kera), Lumican (Lum), aldehyde dehydrogenase 3 family member A1 (Aldh3a1) and Aquaporin 1 (Aqp1) of differentiated keratocytes. Moreover, immunohistochemistry illustrated the positive staining of tumor protein P63 (P63), aldehyde dehydrogenase 3 family member A1 (Aldh3a1), lumican (Lum) and collagen I (Col-I), which are considerable for native cornea. This study manifested a feasible platform to construct tissue-engineered canine cornea for functional grafts and positively contributed to the body of knowledge related to canine corneal stem cells.
      Competing Interests: The authors have declared that no competing interests exist.
    • References:
      Eye (Lond). 1990;4 ( Pt 3):389-424. (PMID: 2209904)
      Nat Protoc. 2011 Sep 22;6(10):1612-31. (PMID: 21959241)
      J Tissue Eng Regen Med. 2018 Jan;12(1):285-295. (PMID: 28600807)
      Science. 2010 Jul 30;329(5991):528-31. (PMID: 20671180)
      Stem Cells. 2005 Feb;23(2):288-96. (PMID: 15671151)
      Biomaterials. 2011 Aug;32(22):5086-91. (PMID: 21501866)
      Curr Eye Res. 2013 Jan;38(1):27-34. (PMID: 23051105)
      Biomater Sci. 2018 May 29;6(6):1492-1502. (PMID: 29624196)
      Invest Ophthalmol Vis Sci. 2018 Jul 2;59(8):3728-3738. (PMID: 30046814)
      Sci Transl Med. 2014 Dec 10;6(266):266ra172. (PMID: 25504883)
      Tissue Eng Part A. 2013 Sep;19(17-18):2063-75. (PMID: 23557404)
      Sci Rep. 2015 Jun 03;5:10839. (PMID: 26039975)
      Indian J Ophthalmol. 2012 Sep-Oct;60(5):423-7. (PMID: 22944753)
      Stem Cells Dev. 2020 Apr 1;29(7):425-439. (PMID: 31973649)
      Tissue Eng Part B Rev. 2015 Jun;21(3):278-87. (PMID: 25434371)
      Cells. 2019 Apr 12;8(4):. (PMID: 31013745)
      Sci Rep. 2019 Jul 5;9(1):9763. (PMID: 31278274)
      Biomaterials. 2014 Apr;35(13):4005-14. (PMID: 24508079)
      Clin Exp Ophthalmol. 2007 Mar;35(2):140-7. (PMID: 17362455)
      World J Stem Cells. 2014 Sep 26;6(4):391-403. (PMID: 25258661)
      Biomaterials. 2012 May;33(13):3529-38. (PMID: 22336295)
      Biomaterials. 2017 Jan;112:1-9. (PMID: 27741498)
      Tissue Eng Part C Methods. 2011 May;17(5):537-48. (PMID: 21175372)
      Biomaterials. 2009 Mar;30(7):1299-308. (PMID: 19059642)
      Invest Ophthalmol Vis Sci. 2011 Feb 09;52(2):731-6. (PMID: 20926820)
      J Cataract Refract Surg. 2011 Mar;37(3):588-98. (PMID: 21333881)
      Invest Ophthalmol Vis Sci. 2002 Jul;43(7):2114-21. (PMID: 12091405)
      J Mater Sci Mater Med. 2007 Feb;18(2):195-200. (PMID: 17323150)
      Vet Res Commun. 2009 Sep;33 Suppl 1:169-71. (PMID: 19655266)
      FASEB J. 2005 Aug;19(10):1371-3. (PMID: 15901670)
      Cytotherapy. 2006;8(4):315-7. (PMID: 16923606)
      Bull World Health Organ. 2001;79(3):214-21. (PMID: 11285665)
      J Dig Dis. 2012 Mar;13(3):168-178. (PMID: 22356312)
      Exp Eye Res. 2007 Jan;84(1):3-12. (PMID: 16797007)
      Exp Eye Res. 2010 Feb;90(2):350-9. (PMID: 20025872)
      Exp Eye Res. 2009 Aug;89(2):159-65. (PMID: 19298815)
      PLoS One. 2014 Apr 04;9(4):e93012. (PMID: 24705327)
      Sci Transl Med. 2010 Aug 25;2(46):46ra61. (PMID: 20739681)
      J Surfactants Deterg. 2019 Sep;22(5):1119-1127. (PMID: 32336911)
      Biomaterials. 2008 Aug-Sep;29(24-25):3415-28. (PMID: 18502501)
      PLoS One. 2015 Mar 03;10(3):e0118517. (PMID: 25734654)
      Stem Cells. 2005 Oct;23(9):1266-75. (PMID: 16051989)
      Glycoconj J. 2002 May-Jun;19(4-5):275-85. (PMID: 12975606)
      Vet Ophthalmol. 2015 Jan;18(1):59-68. (PMID: 24471763)
      Membranes (Basel). 2021 Oct 27;11(11):. (PMID: 34832052)
      Am J Respir Cell Mol Biol. 1997 Jan;16(1):62-8. (PMID: 8998080)
      Int J Nanomedicine. 2011;6:1509-15. (PMID: 21845040)
      Invest Ophthalmol Vis Sci. 1986 Oct;27(10):1470-7. (PMID: 3531080)
      Proc Natl Acad Sci U S A. 2005 Jul 5;102(27):9523-8. (PMID: 15983386)
      Braz J Med Biol Res. 2003 Aug;36(8):1037-46. (PMID: 12886457)
      Canine Genet Epidemiol. 2017 Jun 15;4:5. (PMID: 28630713)
      Biomed Mater. 2018 Mar 06;13(3):032002. (PMID: 29021411)
      J Orthop Res. 2018 Feb;36(2):808-815. (PMID: 29027711)
      Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2011 Jul;27(7):736-9. (PMID: 21722523)
      Prog Retin Eye Res. 2001 Sep;20(5):625-37. (PMID: 11470453)
      Indian J Ophthalmol. 2018 Feb;66(2):190-194. (PMID: 29380756)
      Biomaterials. 2014 Apr;35(12):3744-55. (PMID: 24503156)
      Biomacromolecules. 2011 May 9;12(5):1686-96. (PMID: 21425769)
      Biomaterials. 2005 Feb;26(4):433-41. (PMID: 15275817)
      IEEE Trans Terahertz Sci Technol. 2015 Mar;5(2):170-183. (PMID: 26322247)
      Biomaterials. 2003 Jan;24(2):357-65. (PMID: 12419638)
      Curr Opin Cell Biol. 2012 Oct;24(5):685-94. (PMID: 22835462)
      Biomaterials. 2010 Apr;31(10):2926-33. (PMID: 20044136)
      Exp Eye Res. 2020 Aug;197:108090. (PMID: 32522478)
      Vet Clin North Am Small Anim Pract. 1997 Sep;27(5):1067-107. (PMID: 9326968)
      Vet Ophthalmol. 2015 Jul;18(4):317-25. (PMID: 25495909)
      Clin Exp Optom. 2015 May;98(3):218-23. (PMID: 25676043)
    • Accession Number:
      0 (ATP Binding Cassette Transporter, Subfamily G, Member 2)
      0 (Biocompatible Materials)
      0 (Collagen Type I)
      0 (Eye Proteins)
      0 (Lumican)
      146410-94-8 (Aquaporin 1)
      9000-70-8 (Gelatin)
      9007-76-5 (Fibroins)
    • Publication Date:
      Date Created: 20220204 Date Completed: 20220316 Latest Revision: 20231103
    • Publication Date:
      20231103
    • Accession Number:
      PMC8815981
    • Accession Number:
      10.1371/journal.pone.0263141
    • Accession Number:
      35120168