Sugar Metabolism and Transcriptome Analysis Reveal Key Sugar Transporters during Camellia oleifera Fruit Development.

Publisher: MDPI Country of Publication: Switzerland NLM ID: 101092791 Publication Model: Electronic Cited Medium: Internet ISSN: 1422-0067 (Electronic) Linking ISSN: 14220067 NLM ISO Abbreviation: Int J Mol Sci Subsets: MEDLINE

Original Publication: Basel, Switzerland : MDPI, [2000-

Gene Expression Profiling* ; Gene Expression Regulation, Plant* ; Transcriptome*; Camellia/*physiology ; Fruit/*physiology ; Monosaccharide Transport Proteins/*genetics ; Sugars/*metabolism; Carbohydrate Metabolism ; Computational Biology/methods ; High-Throughput Nucleotide Sequencing ; Molecular Sequence Annotation ; Monosaccharide Transport Proteins/metabolism ; Organ Specificity ; Plant Development/genetics

Camellia oleifera is a widely planted woody oil crop with economic significance because it does not occupy cultivated land. The sugar-derived acetyl-CoA is the basic building block in fatty acid synthesis and oil synthesis in C. oleifera fruit; however, sugar metabolism in this species is uncharacterized. Herein, the changes in sugar content and metabolic enzyme activity and the transcriptomic changes during C. oleifera fruit development were determined in four developmental stages (CR6: young fruit formation; CR7: expansion; CR9: oil transformation; CR10: ripening). CR7 was the key period of sugar metabolism since it had the highest amount of soluble sugar, sucrose, and glucose with a high expression of genes related to sugar transport (four sucrose transporters (SUTs) or and one SWEET-like gene, also known as a sugar, will eventually be exported transporters) and metabolism. The significant positive correlation between their expression and sucrose content suggests that they may be the key genes responsible for sucrose transport and content maintenance. Significantly differentially expressed genes enriched in the starch and sucrose metabolism pathway were observed in the CR6 versus CR10 stages according to KEGG annotation. The 26 enriched candidate genes related to sucrose metabolism provide a molecular basis for further sugar metabolism studies in C. oleifera fruit.

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Keywords: Camellia oleifera; RNA-seq; enzymes in sucrose metabolism; fruit development; sucrose transport; sugar content

0 (Monosaccharide Transport Proteins)
0 (Sugars)

Date Created: 20220121 Date Completed: 20220203 Latest Revision: 20220203

20231215

PMC8775869

10.3390/ijms23020822

35055010