Proteomic and lipidomics analyses of high fatty acid AhDGAT3 transgenic soybean reveals the key lipase gene associated with the lipid internal mechanism.

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  • Additional Information
    • Source:
      Publisher: Canadian Science Publishing Country of Publication: Canada NLM ID: 8704544 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1480-3321 (Electronic) Linking ISSN: 08312796 NLM ISO Abbreviation: Genome Subsets: MEDLINE
    • Publication Information:
      Publication: 2011- : Ottawa, ON : Canadian Science Publishing
      Original Publication: Ottawa, Canada : National Research Council of Canada, c1987-
    • Subject Terms:
      Fatty Acids*/metabolism ; Glycine max*/genetics ; Glycine max*/metabolism; Humans ; Lipase/analysis ; Lipase/metabolism ; Lipidomics ; Plants, Genetically Modified/genetics ; Proteomics ; Seeds/genetics
    • Abstract:
      Vegetable oil is one of the most important components of human nutrition. Soybean ( Glycine max ) is an important oil crop worldwide and contains rich unsaturated fatty acids. Diacylglycerol acyltransferase (DGAT) is a key rate-limiting enzyme in the Kennedy pathway from diacylglycerol (DAG) to triacylglycerol (TAG). In this study, we conducted further research using T 3 AhDGAT3 transgenic soybean. A high-performance gas chromatography flame ionization detector showed that oleic acid (18:1) content and total fatty acid content of transgenic soybean were significantly higher than those of the wild type (WT). However, linoleic acid (18:2) was much lower than that in the WT. For further mechanistic studies, 20 differentially expressed proteins (DEPs) and 119 differentially expressed metabolites (DEMs) were identified between WT (JACK) and AhDGAT3 transgenic soybean mature seeds using proteomic and lipidomics analyses. Combined proteomic and lipidomics analyses showed that the upregulation of the key DEP (lipase GDSL domain-containing protein) in lipid transport and metabolic process induced an increase in the total fatty acid and 18:1 composition, but a decrease in the 18:2 composition of fatty acids. Our study provides new insights into the deep study of molecular mechanism underlying the enhancement of fatty acids in transgenic soybeans, especially oleic acid and total fatty acid, which are enhanced by over-expression of AhDGAT3 .
    • Contributed Indexing:
      Keywords: AhDGAT3 gene; fatty acid content; gène AhDGAT3; lipidomics; lipidomique; proteomic; protéomique; soja transgénique; teneur en acides gras; transgenic soybean
    • Accession Number:
      0 (Fatty Acids)
      EC 3.1.1.3 (Lipase)
    • Publication Date:
      Date Created: 20220107 Date Completed: 20220303 Latest Revision: 20231213
    • Publication Date:
      20231215
    • Accession Number:
      10.1139/gen-2021-0043
    • Accession Number:
      34995159