Alphaherpesvirus-induced activation of plasmacytoid dendritic cells depends on the viral glycoprotein gD and is inhibited by non-infectious light particles.

Item request has been placed! ×
Item request cannot be made. ×
loading   Processing Request
Read Online Read More Add to Saved list
  • Additional Information
    • Source:
      Publisher: Public Library of Science Country of Publication: United States NLM ID: 101238921 Publication Model: eCollection Cited Medium: Internet ISSN: 1553-7374 (Electronic) Linking ISSN: 15537366 NLM ISO Abbreviation: PLoS Pathog Subsets: MEDLINE
    • Publication Information:
      Original Publication: San Francisco, CA : Public Library of Science, c2005-
    • Subject Terms:
      Dendritic Cells/*immunology ; Herpes Simplex/*immunology ; Interferon Type I/*metabolism ; Pseudorabies/*immunology ; Viral Envelope Proteins/*metabolism ; Virion/*physiology; Animals ; Dendritic Cells/metabolism ; Dendritic Cells/virology ; Herpes Simplex/metabolism ; Herpes Simplex/virology ; Herpesvirus 1, Human/physiology ; Herpesvirus 1, Suid/physiology ; Male ; Pseudorabies/metabolism ; Pseudorabies/virology ; Swine ; Testis/immunology ; Testis/metabolism ; Testis/virology ; Viral Envelope Proteins/genetics
    • Abstract:
      Plasmacytoid dendritic cells (pDC) are important innate immune cells during the onset of viral infections as they are specialized in the production of massive amounts of antiviral type I interferon (IFN). Alphaherpesviruses such as herpes simplex virus (HSV) or pseudorabies virus (PRV) are double stranded DNA viruses and potent stimulators of pDC. Detailed information on how PRV activates porcine pDC is lacking. Using PRV and porcine primary pDC, we report here that PRV virions, so-called heavy (H-)particles, trigger IFNα production by pDC, whereas light (L-) particles that lack viral DNA and capsid do not. Activation of pDC requires endosomal acidification and, importantly, depends on the PRV gD envelope glycoprotein and O-glycosylations. Intriguingly, both for PRV and HSV-1, we found that L-particles suppress H-particle-mediated activation of pDC, a process which again depends on viral gD. This is the first report describing that gD plays a critical role in alphaherpesvirus-induced pDC activation and that L-particles directly interfere with alphaherpesvirus-induced IFNα production by pDC.
      Competing Interests: The authors have declared that no competing interests exist.
    • References:
      J Virol. 1986 Aug;59(2):216-23. (PMID: 3016293)
      Nat Med. 1999 Aug;5(8):919-23. (PMID: 10426316)
      J Exp Med. 2005 Aug 1;202(3):425-35. (PMID: 16061729)
      J Virol. 2006 Jul;80(13):6387-98. (PMID: 16775327)
      Virology. 2001 Mar 15;281(2):315-28. (PMID: 11277703)
      J Virol. 2006 Jul;80(13):6235-46. (PMID: 16775311)
      Vet Immunol Immunopathol. 2002 Sep 25;88(3-4):209-16. (PMID: 12127418)
      Arch Virol. 1979;60(1):13-23. (PMID: 226030)
      Arch Virol. 1992;123(3-4):425-49. (PMID: 1562239)
      J Gen Virol. 1992 Feb;73 ( Pt 2):269-76. (PMID: 1311356)
      Int Immunol. 2008 Jan;20(1):45-56. (PMID: 18000008)
      Blood. 2003 Oct 1;102(7):2513-21. (PMID: 12829599)
      J Immunol Methods. 1994 Apr 15;170(2):233-45. (PMID: 8158001)
      J Virol. 1987 Sep;61(9):2764-9. (PMID: 3039168)
      Front Immunol. 2020 Jul 07;11:1429. (PMID: 32733474)
      Dev Comp Immunol. 2014 May;44(1):206-16. (PMID: 24382335)
      J Clin Virol. 2004 Aug;30(4):329-36. (PMID: 15163423)
      J Exp Med. 2002 Feb 18;195(4):517-28. (PMID: 11854364)
      Blood. 2008 Apr 15;111(8):4245-53. (PMID: 18258799)
      Virology. 1994 Oct;204(1):1-7. (PMID: 8091644)
      Arch Virol. 1995;140(6):1137-46. (PMID: 7611884)
      Eur J Immunol. 2008 Jul;38(7):1822-32. (PMID: 18581320)
      J Virol. 2011 May;85(9):4297-308. (PMID: 21307187)
      Viruses. 2009 Dec;1(3):383-419. (PMID: 21994554)
      Virology. 1997 Dec 22;239(2):378-88. (PMID: 9434728)
      Nat Immunol. 2004 Dec;5(12):1219-26. (PMID: 15549123)
      Virology. 1959 Apr;7(4):394-407. (PMID: 13669311)
      Mol Cell Proteomics. 2015 Sep;14(9):2341-56. (PMID: 25693804)
      J Gen Virol. 1985 Dec;66 ( Pt 12):2781-6. (PMID: 2999320)
      J Virol. 2015 Nov;89(21):11046-55. (PMID: 26311871)
      Hybridoma. 1991 Feb;10(1):35-47. (PMID: 2032734)
      Virology. 1998 Jun 20;246(1):179-89. (PMID: 9657005)
      Science. 2006 Oct 13;314(5797):308-12. (PMID: 16973841)
      Front Microbiol. 2020 Sep 29;11:1997. (PMID: 33117298)
      J Virol. 2009 Feb;83(4):1952-61. (PMID: 19073735)
      Science. 2007 Mar 9;315(5817):1398-401. (PMID: 17272685)
      Vet Immunol Immunopathol. 2010 May 15;135(1-2):20-33. (PMID: 19939462)
      Proc Natl Acad Sci U S A. 2007 Feb 27;104(9):3396-401. (PMID: 17360657)
      Front Microbiol. 2017 Dec 19;8:2565. (PMID: 29312245)
      Pathogens. 2020 Oct 27;9(11):. (PMID: 33121171)
      PLoS Pathog. 2019 Nov 1;15(11):e1008087. (PMID: 31675371)
      Adv Virus Res. 2019;104:225-281. (PMID: 31439150)
      Virology. 2014 Jun;458-459:83-92. (PMID: 24928041)
      Cell Rep. 2020 Sep 1;32(9):108099. (PMID: 32877681)
      Immunity. 2008 Sep 19;29(3):352-61. (PMID: 18799143)
      J Immunol. 2008 Jun 1;180(11):7525-36. (PMID: 18490753)
      Proc Natl Acad Sci U S A. 2004 Aug 3;101(31):11416-21. (PMID: 15272082)
      J Virol. 1999 Oct;73(10):8019-26. (PMID: 10482550)
      J Virol. 2003 May;77(10):5657-67. (PMID: 12719558)
      Eur J Immunol. 2011 Apr;41(4):1014-23. (PMID: 21413003)
      Science. 1999 Jun 11;284(5421):1835-7. (PMID: 10364556)
      Virology. 1992 Oct;190(2):682-8. (PMID: 1325700)
      J Immunol. 1984 Jul;133(1):368-75. (PMID: 6609988)
      J Immunol. 2006 Dec 1;177(11):7510-4. (PMID: 17114418)
      J Virol. 1997 Oct;71(10):7687-95. (PMID: 9311852)
      Virology. 1998 Nov 25;251(2):317-26. (PMID: 9837796)
      Front Immunol. 2019 Apr 12;10:778. (PMID: 31031767)
      J Gen Virol. 1991 Mar;72 ( Pt 3):661-8. (PMID: 1848601)
      PLoS Pathog. 2013 Oct;9(10):e1003728. (PMID: 24204273)
      Dev Comp Immunol. 2016 Dec;65:352-357. (PMID: 27524460)
      Nat Immunol. 2000 Oct;1(4):305-10. (PMID: 11017101)
      J Gen Virol. 1996 Sep;77 ( Pt 9):2277-85. (PMID: 8811028)
      Blood. 2006 Mar 15;107(6):2423-31. (PMID: 16293610)
      PLoS One. 2010 Mar 05;5(3):e9560. (PMID: 20221426)
      J Virol. 2000 Dec;74(23):10863-72. (PMID: 11069980)
      Proc Soc Exp Biol Med. 1964 Mar;115:814-6. (PMID: 14155835)
      Front Immunol. 2020 Aug 26;11:1970. (PMID: 32983130)
      J Virol. 2010 Apr;84(8):4060-72. (PMID: 20130046)
      J Virol. 2008 Mar;82(6):2821-35. (PMID: 18184710)
      Nat Immunol. 2001 Dec;2(12):1144-50. (PMID: 11713464)
      J Virol. 2001 Nov;75(22):10856-69. (PMID: 11602726)
      J Virol. 2011 Jan;85(2):895-904. (PMID: 20980519)
      J Immunol. 2004 Jun 15;172(12):7485-94. (PMID: 15187127)
      Nat Rev Immunol. 2004 Jul;4(7):499-511. (PMID: 15229469)
      J Virol. 2017 Mar 13;91(7):. (PMID: 28122975)
      Microbiol Mol Biol Rev. 2005 Sep;69(3):462-500. (PMID: 16148307)
      Blood. 2006 Mar 1;107(5):2022-9. (PMID: 16269620)
      Front Microbiol. 2019 Nov 26;10:2627. (PMID: 31849849)
      Immunology. 2004 May;112(1):28-37. (PMID: 15096181)
      Science. 1998 Jun 5;280(5369):1618-20. (PMID: 9616127)
      J Virol. 2017 Sep 12;91(19):. (PMID: 28747498)
      Proc Natl Acad Sci U S A. 2014 Nov 11;111(45):16118-23. (PMID: 25352670)
      J Exp Med. 2003 Aug 4;198(3):513-20. (PMID: 12900525)
      Eur J Immunol. 2006 Jul;36(7):1674-83. (PMID: 16783856)
      J Immunol. 2007 Sep 15;179(6):4219-30. (PMID: 17785862)
      J Virol. 1991 Oct;65(10):5348-56. (PMID: 1654444)
      Virology. 1995 Aug 1;211(1):209-17. (PMID: 7645212)
      J Gen Virol. 1999 May;80 ( Pt 5):1283-1291. (PMID: 10355775)
      J Virol. 2018 Oct 29;92(22):. (PMID: 30185590)
      J Virol. 2000 Aug;74(15):7137-45. (PMID: 10888654)
      J Immunol. 2008 Nov 15;181(10):6747-56. (PMID: 18981092)
      Immunobiology. 2006;211(6-8):525-35. (PMID: 16920491)
      Virology. 1997 Oct 13;237(1):113-22. (PMID: 9344913)
      Immunol Rev. 2010 Mar;234(1):142-62. (PMID: 20193017)
      Nat Med. 2002 Apr;8(4):392-8. (PMID: 11927946)
      Vet Microbiol. 2007 Jan 31;119(2-4):115-20. (PMID: 17084564)
      Virology. 2005 Jan 5;331(1):144-50. (PMID: 15582661)
      J Gen Virol. 1992 Feb;73 ( Pt 2):277-84. (PMID: 1311357)
    • Accession Number:
      0 (Interferon Type I)
      0 (Viral Envelope Proteins)
      0 (glycoprotein gD, herpes simplex virus type 1)
    • Publication Date:
      Date Created: 20211129 Date Completed: 20211231 Latest Revision: 20211231
    • Publication Date:
      20240829
    • Accession Number:
      PMC8659615
    • Accession Number:
      10.1371/journal.ppat.1010117
    • Accession Number:
      34843605