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Dysregulated Arginine Metabolism in Young Patients with Chronic Persistent Asthma and in Human Bronchial Epithelial Cells.
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- Additional Information
- Source:
Publisher: MDPI Publishing Country of Publication: Switzerland NLM ID: 101521595 Publication Model: Electronic Cited Medium: Internet ISSN: 2072-6643 (Electronic) Linking ISSN: 20726643 NLM ISO Abbreviation: Nutrients Subsets: MEDLINE
- Publication Information:
Original Publication: Basel, Switzerland : MDPI Publishing
- Subject Terms:
- Abstract:
Background: Recent metabolomics studies have found circulatory metabolism alterations in patients with asthma, indicating that altered metabolites played a significant role in asthma. However, the regulatory mechanisms in asthma, especially in young chronic persistent asthma remain underexplored.
Methods: In this study, a prospective cohort of 162 patients diagnosed of asthma admitted to the First Affiliated Hospital of Xi'an Jiaotong University from January 2018 to December 2019 was used to perform a nested case-control study. Among them, we included 30 patients with chronic persistent asthma between 20 to 35 years old; 30 health control with evenly distributed age and sex were then recruited. Nontargeted metabolomics was applied to identify serum metabolic profiles and altered metabolic pathways.
Results: In vitro, human bronchial epithelial cells (HBECs) line BEAS-2B with the addition of L-citrulline and/or asymmetric dimethylarginine (ADMA) model was utilized and the concentrations of nitric oxide (NO) metabolites were tested to evaluate the therapeutic potential of L-citrulline. The young patients with chronic persistent asthma displayed dysregulated serum metabolic profiles, especially enriched in arginine metabolism. The ratio of L-citrulline to ornithine is associated with blood eosinophil count. In vitro, adding L-citrulline could reverse ADMA-mediated reduction of NOx at lower L-arginine concentration (25 μM), but was ineffective in the higher L-arginine concentration (100 μM) media.
Conclusions: The arginine metabolism balance is of vital importance during the pathogenesis and progression of chronic asthma. L-citrulline could be a powerful approach to restore airway NO production, potentially exhibiting therapeutic benefits among young patients with chronic asthma.
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- Grant Information:
81800390 National Natural Science Foundation of China; 2020KW-049, 2021KWZ-25, S2020-YF-GHMS-0014, 2017SF-085, 2020GXLH-Y-015 Key Research and Development Program of Shaanxi; XJTU1AF-CRF-2018-025 the Clinical Research Award of the First Affiliated Hospital of Xi'an Jiaotong University
- Contributed Indexing:
Keywords: L-citrulline; arginine metabolism; chronic persistent asthma; human airway epithelial cells
- Accession Number:
29VT07BGDA (Citrulline)
31C4KY9ESH (Nitric Oxide)
63CV1GEK3Y (N,N-dimethylarginine)
94ZLA3W45F (Arginine)
E524N2IXA3 (Ornithine)
- Publication Date:
Date Created: 20211127 Date Completed: 20211203 Latest Revision: 20211214
- Publication Date:
20231215
- Accession Number:
PMC8622016
- Accession Number:
10.3390/nu13114116
- Accession Number:
34836371
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