The utility of 'home-made' reagent red blood cells for antibody screening during pre-transfusion compatibility testing in Uganda.

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  • Additional Information
    • Source:
      Publisher: Faculty of Medicine, Makerere University Country of Publication: Uganda NLM ID: 101149451 Publication Model: Print Cited Medium: Internet ISSN: 1729-0503 (Electronic) Linking ISSN: 16806905 NLM ISO Abbreviation: Afr Health Sci Subsets: MEDLINE
    • Publication Information:
      Original Publication: Kampala, Uganda : Faculty of Medicine, Makerere University, [2001-
    • Subject Terms:
      Blood Transfusion* ; Erythrocytes* ; Indicators and Reagents*; Isoantibodies/*blood; Adult ; Female ; Humans ; Male ; Middle Aged ; Uganda ; Young Adult
    • Abstract:
      Background: The WHO recommends that pre-transfusion testing should include ABO/RhD grouping followed by screening for red blood cell (RBC) alloantibodies using the indirect antiglobulin test (IAT). However, in Uganda, current practice does not include RBC alloantibody screening.
      Objective: To assess the utility of 'home-made' reagent RBCs in alloantibody screening.
      Materials and Methods: In a laboratory-based study, group O RhD positive volunteer donors were recruited and their extended phenotype performed for C, c, E, e, K, Fya, Fyb Jkb, S and s antigens. These 'home-made' reagent RBCs were preserved using Alsever's solution and alloantibody detection tests performed. For quality assurance, repeat alloantibody screening of patients' samples was done at Bloodworks Northwest Laboratory in Seattle, United States.
      Results: A total of 36 group O RhD positive individuals were recruited as reagent RBC donors (median age, 25 years; range, 21 - 58 years; male-to-female ratio, 1.6:1). Out of the 311 IATs performed, 32 (10.3%) were positive. Confirmatory IAT testing in the United States was in agreement with the findings in Uganda.
      Conclusion: Use of 'home-made' reagent RBCs during pre-transfusion testing in Uganda is feasible. We recommend the introduction of pre-transfusion IAT alloantibody screening in Uganda using 'home-made' reagent RBCs to improve transfusion safety.
      (© 2021 Natukunda B et al.)
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    • Contributed Indexing:
      Keywords: Blood transfusion; Pre-transfusion testing; RBC alloantibody screening; Uganda; ‘Home-made’ reagent RBCs
    • Accession Number:
      0 (Indicators and Reagents)
      0 (Isoantibodies)
    • Publication Date:
      Date Created: 20211119 Date Completed: 20211203 Latest Revision: 20220428
    • Publication Date:
      20221213
    • Accession Number:
      PMC8568224
    • Accession Number:
      10.4314/ahs.v21i2.38
    • Accession Number:
      34795736