Molecular detection of dugbe orthonairovirus in cattle and their infesting ticks (Amblyomma and Rhipicephalus (Boophilus)) in Nigeria.

Publisher: Public Library of Science Country of Publication: United States NLM ID: 101291488 Publication Model: eCollection Cited Medium: Internet ISSN: 1935-2735 (Electronic) Linking ISSN: 19352727 NLM ISO Abbreviation: PLoS Negl Trop Dis Subsets: MEDLINE

Original Publication: San Francisco, CA : Public Library of Science

Amblyomma/*virology ; Arachnid Vectors/*virology ; Arboviruses/*genetics ; Cattle Diseases/*virology ; Rhipicephalus/*virology; Amblyomma/physiology ; Animals ; Arachnid Vectors/physiology ; Arboviruses/classification ; Arboviruses/isolation & purification ; Cattle ; Cattle Diseases/transmission ; Female ; Male ; Nigeria ; Phylogeny ; Rhipicephalus/physiology

Dugbe orthonairovirus (DUGV), a tick-borne zoonotic arbovirus, was first isolated in 1964 in Nigeria. For over four decades, no active surveillance was conducted to monitor the spread and genetic variation of DUGV. This study detected and genetically characterized DUGV circulating in cattle and their infesting ticks (Amblyomma and Rhipicephalus (Boophilus)) in Kwara State, North-Central Nigeria. Blood and or ticks were collected from 1051 cattle at 31 sampling sites (abattoirs and farms) across 10 local government areas of the State. DUGV detection was carried out by RT-qPCR, and positive samples sequenced and phylogenetically analysed. A total of 11824 ticks, mostly A. variegatum (36.0%) and R. (B.) microplus (63.9%), were obtained with mean tick burden of 12 ticks/cattle. Thirty-four (32 A. variegatum and two R. (B.) microplus) of 4644 examined ticks were DUGV-positive, whereas all of the cattle sera tested negative for DUGV genome. Whole genome sequence (S, M and L segments) and phylogenetic analyses indicate that the positive samples shared up to 99.88% nucleotide identity with and clustered around the Nigerian DUGV prototype strain IbAr 1792. Hence, DUGV with high similarity to the previously characterised strain has been detected in Nigeria. To our knowledge, this is the first report of DUGV in North-Central Nigeria and the most recent information after its last surveillance in 1974.
Competing Interests: The authors have declared that no competing interests exist.

Am J Trop Med Hyg. 1978 May;27(3):600-4. (PMID: 98066)
Am J Trop Med Hyg. 1970 Nov;19(6):Suppl:1082-4. (PMID: 5491839)
Rev Bras Parasitol Vet. 2016 Jul-Sep;25(3):317-26. (PMID: 27579530)
Epidemiol Infect. 1996 Jun;116(3):353-61. (PMID: 8666081)
Am J Trop Med Hyg. 2016 May 4;94(5):1107-1122. (PMID: 26903607)
Ticks Tick Borne Dis. 2017 Jun;8(4):640-645. (PMID: 28479064)
J Gen Virol. 1974 Jun;23(3):297-307. (PMID: 4365798)
J Med Entomol. 1979 May 22;15(4):307-417. (PMID: 113533)
Emerg Infect Dis. 2009 Feb;15(2):147-54. (PMID: 19193256)
Viruses. 2021 Feb 26;13(3):. (PMID: 33652845)
BMC Bioinformatics. 2018 Aug 29;19(1):307. (PMID: 30157759)
Ann Trop Med Parasitol. 1975 Mar;69(1):49-64. (PMID: 1124969)
Trans R Soc Trop Med Hyg. 1980;74(6):732-7. (PMID: 7210125)
Virus Res. 1991 Nov;21(3):199-212. (PMID: 1767584)
Am J Trop Med Hyg. 1989 Apr;40(4):410-7. (PMID: 2496609)
Mol Biol Evol. 2018 Jun 1;35(6):1547-1549. (PMID: 29722887)
Am J Trop Med Hyg. 1981 Jul;30(4):855-61. (PMID: 7196163)
Parasit Vectors. 2013 Jun 07;6:171. (PMID: 23758913)
Emerg Infect Dis. 2006 Jul;12(7):1074-80. (PMID: 16836823)
J Egypt Public Health Assoc. 1976;51(6):331-7. (PMID: 1025257)
Bioinformatics. 2018 Jul 15;34(14):2490-2492. (PMID: 29506019)
Trans R Soc Trop Med Hyg. 1996 Jan-Feb;90(1):31-3. (PMID: 8730305)
J Gen Virol. 2013 Feb;94(Pt 2):298-307. (PMID: 23136361)

Date Created: 20211117 Date Completed: 20220104 Latest Revision: 20231103

20231215

PMC8598060

10.1371/journal.pntd.0009905

34788303